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Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence
被引：0
|作者：
Xueqian Gong
Shouyi Chen
Jiming Gong
Feng Liu
机构：
[1] Chinese Academy of Sciences,Institute of Genetics
[2] De Montfort University,Chinese Center of the Norman Borlaug Institute for Plant Science Research
来源：
Science in China Series C: Life Sciences
|
1998年
/
41卷
关键词：
rice;
BAC library construction;
TAS;
BAC clone;
RFLP map;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Using single primer (TTTAGGG)3 or (CCCTAAA)3CCC corresponding to rice telomeric repeat sequences, two fragments were amplified from rice genomic DNA and named Tas1 and Tas2. These two fragments did not show polymorphism between the mapping parents. Tasl was found to hybridize to rice telomeric regions byin situ hybridization (FISH). Then a rice bacterial artificial chromosome (BAC) library was constructed and Tasl was used to screen it. One positive clone was identified. A single copy sequence of this BAC clone was mapped at the end of the 6th chromosome of rice.
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页码：617 / 622
页数：5
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