Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence

被引：0
|
作者：
Xueqian Gong
Shouyi Chen
Jiming Gong
Feng Liu
机构：
[1] Chinese Academy of Sciences,Institute of Genetics
[2] De Montfort University,Chinese Center of the Norman Borlaug Institute for Plant Science Research
来源：
Science in China Series C: Life Sciences | 1998年 / 41卷
关键词：
rice; BAC library construction; TAS; BAC clone; RFLP map;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Using single primer (TTTAGGG)3 or (CCCTAAA)3CCC corresponding to rice telomeric repeat sequences, two fragments were amplified from rice genomic DNA and named Tas1 and Tas2. These two fragments did not show polymorphism between the mapping parents. Tasl was found to hybridize to rice telomeric regions byin situ hybridization (FISH). Then a rice bacterial artificial chromosome (BAC) library was constructed and Tasl was used to screen it. One positive clone was identified. A single copy sequence of this BAC clone was mapped at the end of the 6th chromosome of rice.
引用
收藏
页码：617 / 622
页数：5
相关论文
共 50 条
  • [1] Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence
    巩学千
    陈受宜
    龚继明
    刘峰
    Science in China(Series C:Life Sciences), 1998, (06) : 617 - 622
  • [2] Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence
    Gong, XQ
    Chen, SY
    Gong, JM
    Liu, F
    SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 1998, 41 (06): : 617 - 622
  • [3] Bacterial artificial chromosome (BAC) clones and the current clone map of the zebrafish genome
    Koch, R
    Rauch, GJ
    Humphray, S
    Geisler, R
    Plasterk, R
    ZEBRAFISH:2ND EDITION GENETICS GENOMICS AND INFORMATICS, 2004, 77 : 295 - 304
  • [4] Isolation and characterization of five rice telomere-associated sequences
    Gong, XQ
    Hong, DJ
    Chen, SY
    Shen, LS
    Li, P
    Yan, CJ
    SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 1998, 41 (04): : 372 - 380
  • [5] Cloning and genetic mapping of wheat telomere-associated sequences
    Mao, L
    Devos, KM
    Zhu, L
    Gale, MD
    MOLECULAR & GENERAL GENETICS, 1997, 254 (05): : 584 - 591
  • [6] Cloning and genetic mapping of wheat telomere-associated sequences
    L. Mao
    K. M. Devos
    L. Zhu
    M. D. Gale
    Molecular and General Genetics MGG, 1997, 254 : 584 - 591
  • [7] Isolation and characterization of two rice telomere-associated sequences
    Hong, DJ
    Hung, TJ
    Chen, SY
    CHINESE SCIENCE BULLETIN, 1996, 41 (03): : 242 - 246
  • [8] Isolation and characterization of five rice telomere-associated sequences
    巩学千
    洪德军
    陈受宜
    沈利爽
    李平
    严长杰
    Science in China(Series C:Life Sciences) , 1998, (04) : 372 - 380
  • [9] Isolation and characterization of five rice telomere-associated sequences
    Xueqian Gong
    Dejun Hong
    Shouyi Chen
    Lishuang Shen
    Ping Li
    Changjie Yan
    Science in China Series C: Life Sciences, 1998, 41 : 372 - 380
  • [10] Isolation and characterization of two rice telomere-associated sequences
    洪德军
    陈受宜
    ChineseScienceBulletin, 1996, (03) : 242 - 246
12345