Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis

被引：0
作者：
Ying-Chun Miao
Jian-Rong Su
Zhi-Jun Zhang
Hui Li
Jing Luo
Ya-Ping Zhang
机构：
[1] Research Institute of Resource Insects,State Key Laboratory of Genetic Resources and Evolution
[2] Chinese Academy of Forest (CAF),Laboratory for Conservation and Utilization of Bio
[3] Kunming Institute of Zoology,resource
[4] Chinese Academy of Sciences,School of Life Science
[5] Yunnan University,undefined
[6] Yunnan University,undefined
来源：
Conservation Genetics | 2008年 / 9卷
关键词：
SSR-anchored PCR; Microsatellite marker; SSR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Eleven polymorphic microsatellite loci were characterized for the endangered conifer Taxus yunnanensis. Eight loci were isolated through SSR-anchored PCR, one locus was developed by cross-species amplification tests, while the last two loci were obtained from cross-species microsatellite sequences available in GenBank. Variability of these markers was tested in 48 individuals collected from its main distribution range in Southwest China. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity varied from 0.000 to 0.625 and from 0.062 to 0.853, respectively. Ten of these eleven loci were significantly deviated from Hardy–Weinberg expectation, except TS07 which showed a distinct heterozygote excess. The availability of these new polymorphic microsatellite markers will provide an ideal marker system for detailed population genetics studies in T. yunnanensis and potentially also for closely related species.
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页码：1683 / 1685
页数：2
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