ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection

被引：185

作者：

Hachim, Asmaa ^{[1
]}

Kavian, Niloufar ^{[1
,2
,3
]}

Cohen, Carolyn A. ^{[1
]}

Chin, Alex W. H. ^{[4
]}

Chu, Daniel K. W. ^{[4
]}

Mok, Chris K. P. ^{[1
]}

Tsang, Owen T. Y. ^{[5
]}

Yeung, Yiu Cheong ^{[5
]}

Perera, Ranawaka A. P. M. ^{[4
]}

Poon, Leo L. M. ^{[1
,4
]}

Peiris, J. S. Malik ^{[1
,4
]}

Valkenburg, Sophie A. ^{[1
]}

机构：

[1] Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, HKU Pasteur Res Pole, Hong Kong, Peoples R China

[2] Univ Paris 05, Ctr Hosp Univ Cochin, AP HP, Fac Med,Sorbonne Paris Cite,Hop Univ Paris Ctr, Paris, France

[3] Univ Paris 05, Inst Cochin, INSERM U1016, Sorbonne Paris Cite, Paris, France

[4] Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, Div Publ Hlth Lab Sci, Hong Kong, Peoples R China

[5] Princess Margaret Hosp, Hosp Author Hong Kong, Infect Dis Ctr, Hong Kong, Peoples R China

来源：

NATURE IMMUNOLOGY | 2020年 / 21卷 / 10期

关键词：

D O I：

10.1038/s41590-020-0773-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Accurate serology testing is urgently needed to help diagnose SARS-CoV-2 infection. Here Valkenburg and colleagues use a luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19 and find ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity The SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.

引用

页码：1293 / +

页数：18

共 28 条

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