Interaction of Cholera Toxin B Subunit with Rat Intestinal Epithelial Cells

被引:0
|
作者
E. V. Navolotskaya
V. B. Sadovnikov
D. V. Zinchenko
V. I. Vladimirov
Y. A. Zolotarev
机构
[1] Russian Academy of Sciences,Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
[2] Pushchino State Natural Science Institute,Institute of Molecular Genetics
[3] Russian Academy of Sciences,undefined
来源
Russian Journal of Bioorganic Chemistry | 2018年 / 44卷
关键词
peptides; receptors; cholera toxin B subunit; thymosin-α; interferon-α;
D O I
暂无
中图分类号
学科分类号
摘要
We prepared 125I-labeled cholera toxin B subunit (125I-labeled CT-B, specific activity 98 Ci/mmol) and found that its binding to rat IEC-6 intestinal epithelial cells was high-affinity (Kd 1.9 nM). The binding of labeled protein was completely inhibited by unlabeled thymosin-α1 (TM-α1), interferon-α2 (IFN-α2), and synthetic peptide LKEKK, which corresponds to residues 16–20 in TM-α1 and 131–135 in IFN-α2 (Ki 1.2, 0.9, and 1.6 nM, respectively), but was not inhibited by synthetic peptide KKEKL with inverted amino acid sequence (Ki > 10 μM). Thus, TM-α1, IFN-α2, and the LKEKK peptide bind with high affinity and specificity to CT-B receptor on rIEC-6 cells. It was found that CT-B and the LKEKK peptide at concentrations of 10–1000 nM increased nitric oxide production and soluble guanylate cyclase activity in the cells in a dose-dependent manner.
引用
收藏
页码:403 / 407
页数:4
相关论文
共 50 条
  • [41] Cholera Toxin Decreases SLC26A3 Expression and Function in Intestinal Epithelial Cells
    Anbazhagan, Arivarasu Natarajan
    Coffing, Hayley
    Priyamvada, Shubha
    Kumar, Anoop
    Alrefai, Waddah A.
    Saksena, Seema
    Gill, Ravinder K.
    Borthakur, Alip
    Dudeja, Pradeep K.
    FASEB JOURNAL, 2018, 32 (01):
  • [42] ENTRY OF CHOLERA-TOXIN (CT) INTO INTESTINAL EPITHELIAL-CELLS - ROLE OF VESICULAR TRAFFIC
    LENCER, WI
    DEALMEIDA, JB
    AUSIELLO, DA
    MADARA, JL
    CLINICAL RESEARCH, 1993, 41 (02): : A263 - A263
  • [43] The role of lacto-series glycosphingolipids in cholera toxin intoxication of host intestinal epithelial cells
    Ghorashi, Atossa
    Kohler, Jennifer
    GLYCOBIOLOGY, 2021, 31 (12) : 1705 - 1705
  • [44] SUBUNIT STRUCTURE OF CHOLERA TOXIN
    LONNROTH, I
    HOLMGREN, J
    JOURNAL OF GENERAL MICROBIOLOGY, 1973, 76 (JUN): : 417 - 427
  • [45] Role of Helicobacter pullorum cytolethal distending toxin subunit B in a model of co-cultured intestinal epithelial cells
    Varon, C.
    Morate, C.
    Richard, S.
    Laharie, D.
    Megraud, F.
    Menard, A.
    ZOONOSES AND PUBLIC HEALTH, 2007, 54 : 92 - 92
  • [46] DETERMINATION OF PRIMARY STRUCTURE OF CHOLERA TOXIN-B SUBUNIT
    LAI, CY
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1977, 252 (20) : 7249 - 7256
  • [47] Expression of Cholera Toxin B Subunit in Transgenic Rice Endosperm
    Oszvald, Maria
    Kang, Tae-Jin
    Tomoskozi, Sandor
    Jenes, Barnabas
    Kim, Tae-Geum
    Cha, Youn-Soo
    Tamas, Laszlo
    Yang, Moon-Sik
    MOLECULAR BIOTECHNOLOGY, 2008, 40 (03) : 261 - 268
  • [48] Expression of Cholera Toxin B Subunit in Transgenic Rice Endosperm
    Maria Oszvald
    Tae-Jin Kang
    Sandor Tomoskozi
    Barnabas Jenes
    Tae-Geum Kim
    Youn-Soo Cha
    Laszlo Tamas
    Moon-Sik Yang
    Molecular Biotechnology, 2008, 40
  • [49] Heterologous expression of cholera toxin B subunit in Saccharomyces cerevisiae
    Jung-Gu Lim
    Hyo-Sang Jin
    Biotechnology and Bioprocess Engineering, 2008, 13 : 598 - 605
  • [50] Cholera Toxin B: One Subunit with Many Pharmaceutical Applications
    Baldauf, Keegan J.
    Royal, Joshua M.
    Hamorsky, Krystal Teasley
    Matoba, Nobuyuki
    TOXINS, 2015, 7 (03): : 974 - 996