T-cell oncogene rhombotin-2 interacts with retinoblastoma-binding protein 2

被引:0
|
作者
Shifeng Mao
Geoffrey AM Neale
Rakesh M Goorha
机构
[1] St Jude Children's Research Hospital,Departments of Virology and Molecular Biology
[2] University of Tennessee,Department of Pathology
[3] Memphis Tennessee,undefined
来源
Oncogene | 1997年 / 14卷
关键词
oncogene; rhombotin-2; RBP2; transcription;
D O I
暂无
中图分类号
学科分类号
摘要
The LIM domain protein rhombotin-2 (RBTN-2/TTG-2/Lmo2) has distinct functions in erythropoiesis and in T-cell leukemogenesis. Additional functions for RBTN2 are indicated by its expression in non-hematopoietic tissues. These diverse functions of RBTN2 are presumed to be accomplished through physical interaction with different protein partners that bind the LIM domains of RBTN2. To identify these proteins which may modulate the activity of RBTN2, a human cDNA library was screened using the yeast two-hybrid assay. Using the RBTN2 LIM domain region as `bait', the retinoblastoma-binding protein 2 (RBP2) was identified as a partner for RBTN2. The interaction between RBTN2 and RBP2 was confirmed using in vitro binding assays, and by co-immunoprecipitation of the two proteins. Deletion analysis showed the second LIM domain of RBTN2 was necessary and sufficient for binding to the last 69 amino acids of RBP2. The interaction between RBTN2 and RBP2 had a functional consequence: the combination of RBP2 and RBTN2 gave higher transcription in vitro, than RBTN2 alone. The interaction with RBP2 suggests two additional functions for RBTN2: (i) RBTN2 may directly affect the activity of RBP2, and/or (ii) RBTN2 may indirectly modulate the functions of the retinoblastoma protein by binding to RBP2.
引用
收藏
页码:1531 / 1539
页数:8
相关论文
共 50 条
  • [31] Re-expression of the retinoblastoma-binding protein 2-homolog 1 reveals tumor-suppressive functions in highly metastatic melanoma cells
    Roesch, Alexander
    Becker, Bernd
    Schneider-Brachert, Wulf
    Hagen, Ilja
    Landthaler, Michael
    Vogt, Thomas
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2006, 126 (08) : 1850 - 1859
  • [32] Transcriptional Repression of the LMO2 Oncogene By Ikaros in T-Cell Acute Lymphoblastic Leukemia
    Ding, Yali
    Song, Chunhua
    Gowda, Chandrika S.
    Kapadia, Malika
    Payne, Kimberly
    Dovat, Sinisa
    BLOOD, 2016, 128 (22)
  • [33] MEF2C as Novel Oncogene for Early T-Cell Precursor (ETP) Leukemia
    Homminga, Irene
    Pieters, Rob
    Langerak, Anton
    de Rooi, Johan
    Stubbs, Andrew
    Verstegen, Monique
    Vuerhard, Maartje
    Buijs-Gladdines, Jessica
    Kooi, Clarissa
    Klous, Petra
    Van Vlierberghe, Pieter
    Ferrando, Adolfo
    Cayuela, Jean-Michel
    Verhaaf, Brenda
    Beverloo, Berna
    Horstmann, Martin A.
    de Haas, Valerie
    Wiekmeijer, Anna-Sophia
    Pike-Overzet, Karin
    Staal, Frank
    de Laat, Woufer
    Soulier, Jean
    Sigaux, Francois
    Meijerink, Jules
    BLOOD, 2010, 116 (21) : 10 - 10
  • [34] Characterization of polycomb repressive complex 2 (PRC2) subunits retinoblastoma-binding protein 4 and 7 (RbBP4/7) in triple-negative breast cancer
    Reed, Rebecca A.
    Lo, Miao-Chia
    Sun, Duxin
    CANCER RESEARCH, 2015, 75
  • [35] Cloning of a T-cell receptor β-chain enhancer binding protein
    Gaikwad, JS
    Fotedar, A
    GENE, 1997, 204 (1-2) : 79 - 83
  • [36] Human T-cell leukemia virus type 2 Tax protein induces interleukin 2-independent growth in a T-cell line
    Rie Kondo
    Masaya Higuchi
    Masahiko Takahashi
    Masayasu Oie
    Yuetsu Tanaka
    Fumitake Gejyo
    Masahiro Fujii
    Retrovirology, 3
  • [37] Human T-cell leukemia virus type 2 Tax protein induces interleukin 2-independent growth in a T-cell line
    Kondo, Rie
    Higuchi, Masaya
    Takahashi, Masahiko
    Oie, Masayasu
    Tanaka, Yuetsu
    Gejyo, Fumitake
    Fujii, Masahiro
    RETROVIROLOGY, 2006, 3 (1)
  • [39] Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-Cell granules
    Andrin, C
    Pinkoski, MJ
    Burns, K
    Atkinson, EA
    Krahenbuhl, O
    Hudig, D
    Fraser, SA
    Winkler, U
    Tschopp, J
    Opas, M
    Bleackley, RC
    Michalak, M
    BIOCHEMISTRY, 1998, 37 (29) : 10386 - 10394
  • [40] ζ-Crystallin is a bcl-2 mRNA binding protein involved in bcl-2 overexpression in T-cell acute lymphocytic leukemia
    Lapucci, Andrea
    Lulli, Matteo
    Amedei, Amedeo
    Papucci, Laura
    Witort, Ewa
    Di Gesualdo, Federico
    Bertolini, Francesco
    Brewer, Gary
    Nicolin, Angelo
    Bevilacqua, Annamaria
    Schiavone, Nicola
    Morello, Dominique
    Donnini, Martino
    Capaccioli, Sergio
    FASEB JOURNAL, 2010, 24 (06): : 1852 - 1865