Genomic and outer membrane protein diversity fingerprints of siderophore producing fluorescent Pseudomonas spp. using RAPD, Rep-PCR and SDS-PAGE profiling

Fluorescent Pseudomonas (FP) is an intrinsic group of plant growth promoting rhizobacteria (PGPR) which also has the potential to control phytopathogens. In the present study sixteen FP isolates were subjected to siderophore production and siderotyping. Four DNA finger printing analysis markers (RAPD, ERIC, BOX A1R, REP and GTG5) were used to assess their genetic diversity through DNA finger printing analysis. Outer membrane protein (OMP) homology of FP was carried out by SDS-PAGE analysis. Chemical characterization showed that the siderophores produced were hydroxamate in nature. Production of siderophores ranged from 21.73 ± 2.18 to 182.03 ± 1.66 μg mL−1 after 72 hr incubation. Maximum production was shown by the isolate M139. Dendrogram obtained from the combined binary data of 10 RAPD primers classified FPs into two distinct clusters. Except for M140, all the isolates from maize rhizosphere were grouped into the same cluster. Among three Rep-PCR analyses, REP primer showed maximum diversity in banding pattern. Combined binary data of three Rep primers also classified FP into two clusters as in RAPD and protein electrophenogram of the OMP. To the best of our knowledge this is the first report on genomic and OMP disparity among hydroxamate producing FPs.