Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis

被引：9
作者：
Carroll P. [1 ]
Muwanguzi-Karugaba J. [1 ]
Parish T. [1 ,2 ]
机构：
[1] Queen Mary University of London, Barts and the London School of Medicine and Dentistry, London
[2] Infectious Disease Research Institute, Seattle, 98102, WA
来源：
BMC Research Notes | / 11卷 / 1期
关键词：
Fluorescent protein; Mycobacteria; Reporter system;
D O I：
10.1186/s13104-018-3798-3
中图分类号：
学科分类号：
摘要：
Objective: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. Results: We generated expression constructs with a full length DsRed under the control of one of three strong, constitutive promoters (Phsp60, PrpsA or PG13) for use in mycobacteria. We confirmed that full length DsRed (225 amino acids) was expressed and fluoresced brightly. In contrast to mCherry, truncated versions of DsRed lacking several amino acids at the N-terminus were not functional. Thus, we have expanded the repertoire of optimized fluorescent proteins for mycobacteria. © 2018 The Author(s).
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