Apocynin and dimethyl sulfoxide synergistically protect against ischemia-reperfusion injury in a rat hind limb ischemia-reperfusion model

被引：2
作者：
Duran A. [1 ]
Ciloglu S. [2 ]
Sehirli O. [3 ]
Yigit A. [2 ]
Buyukdogan H. [2 ]
Duran A. [1 ]
Elcioğlu K. [3 ]
Kabasakal L. [3 ]
机构：
[1] Department of Plastic, Reconstructive and Aesthetic Surgery, Sanliurfa Mehmet Akif Inan Training and Research Hospital, Sanliurfa
[2] Department of Plastic, Reconstructive and Aesthetic Surgery, Haydarpasa Numune Training and Research Hospital, Istanbul
[3] Department of Pharmacology, School of Pharmacy, Marmara University, Istanbul
[4] Department of Dermatology, Sanliurfa Balikligol State Hospital, Sanliurfa
来源：
European Journal of Plastic Surgery | 2017年 / 40卷 / 6期
关键词：
Apocynin; DMSO; Glutathione; IL-1β; Ischemia-reperfusion; Myeloperoxidase; TNF-α;
D O I：
10.1007/s00238-017-1309-8
中图分类号：
学科分类号：
摘要：
Background: Acute ischemia and reperfusion in extremities ignite a complicated inflammatory cycle and lead to damage in the extremities and target tissues. Methods: Rats were randomly divided into four groups. Each experimental group contained eight rats. Group 1 was the sham surgery group. Group 2 was the control I/R group with intraperitoneal serum physiologic (SP) treatment. Group 3 was the I/R group with vehicle treatment (8% DMSO). Group 4 was the I/R group with 20 mg/kg apocynin (vehicle 8% DMSO). The potential protective effects of apocynin and vehicle (DMSO) in the rat hind limb ischemia-reperfusion model were evaluated by measuring the levels of plasma pro-inflammatory cytokines and oxidative stress markers in muscle tissue. Results: The results revealed that both apocynin and vehicle (DMSO) alone increased the antioxidative capacity of ischemic tissue and reduced the levels of plasma proinflammatory cytokines compared to control I/R group. There was a statically more significant effect in the apocynin (vehicle 8% DMSO)-treated group 4. Conclusions: We observed that apocynin and DMSO synergistically prevent the I/R injury in rat hind limb ischemia-reperfusion injury model and meaningfully decrease the inflammatory parameters. Level of Evidence: Not ratable. © 2017, Springer-Verlag Berlin Heidelberg.
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页码：507 / 512
页数：5
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