Sartobind STIC® salt-tolerant membrane chromatography

被引：0
|
作者：
Stefan Fischer-Frühholz
Dave Zhou
Miyako Hirai
机构：
[1] Sartorius Stedim Biotech GmbH,
[2] Sartorius Stedim North America Inc.,undefined
来源：
Nature Methods | 2010年 / 7卷 / 12期
关键词：
D O I：
10.1038/nmeth.f.319
中图分类号：
学科分类号：
摘要：
Polishing in antibody production is usually performed on quaternary ammonium (Q) membranes in flow-through mode, since the overall speed and productivity is much higher than on traditional anion-exchange columns. However, the binding capacity of Q ligands is reduced at high conductivities, so concentrated feed streams must be diluted to adequately remove contaminants. Sartobind STIC PA (primary amine) anion-exchange membrane overcomes this limitation and can be used in high-salt conditions.
引用
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页码：12 / 13
页数：1
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