Synergistic activity of gold nanoparticles with amphotericin B on persister cells of Candida tropicalis biofilms

被引：1
|
作者：
Dasilva, M. A. [1 ,2 ]
Andrada, K. F. Crespo [2 ]
Torales, M. Maldonado [2 ]
Hughes, I. Manrrique [2 ]
Pez, P. [2 ,3 ]
Garcia-Martinez, J. C. [4 ]
Paraje, Maria Gabriela [1 ,2 ]
机构：
[1] Consejo Nacl Invest Cient & Tecn, Inst Multidisciplinario Biol Vegetal IMBIV, Cordoba, Argentina
[2] Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, Catedra Microbiol, X5000HUA, Cordoba, Argentina
[3] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Ciencias Farmaceut, Cordoba, Argentina
[4] Univ Castilla La Mancha, Fac Farm Albacete, Ctr Reg Invest Biomed, Ciudad Real, Spain
来源：
JOURNAL OF NANOBIOTECHNOLOGY | 2024年 / 22卷 / 01期
关键词：
Antibiofilm activity; Gold nanoparticles; Amphotericin B; Synergy; Candida tropicalis; Sessile cells; Persister cells; ALBICANS; AU;
D O I：
10.1186/s12951-024-02415-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim The antifungal activity was studied on sessile and persister cells (PCs) of Candida tropicalis biofilms of gold nanoparticles (AuNPs) stabilized with cetyltrimethylammonium bromide (CTAB-AuNPs) and those conjugated with cysteine, in combination with Amphotericin B (AmB). Materials/methods The PC model was used and synergistic activity was tested by the checkerboard assay. Biofilms were studied by crystal violet and scanning electron microscopy. Results/Conclusions After the combination of both AuNPs and AmB the biofilm biomass was reduced, with significant differences in architecture being observed with a reduced biofilm matrix. In addition, the CTAB-AuNPs-AmB combination significantly reduced PCs. Understanding how these AuNPs aid in the fight against biofilms and the development of new approaches to eradicate PCs has relevance for chronic infection treatment.
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页数：12
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