Conformational study of protein in some species ofFusarium by FT-IR spectroscopy

被引：0
作者：
Jiarong Wan
Yi Liu
Lixin Lan
Yan Gao
Ming Nie
Juan Zhou
机构：
[1] Hunan Chemical Industry Vocational Institute,Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science
[2] Fudan University,Engineering, College
[3] Hunan Agricultural University,undefined
来源：
Annals of Microbiology | 2008年 / 58卷
关键词：
FT-IR spectroscopy; protein secondary structure; amide I band;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel method of estimating conformation of protein secondary structure withinFusarium cells under the influence of growth cultures and bodies was studies by Fourier-Transform Infrared (FT-IR) spectroscopy combined with a deconvolution procedure of amide I band. After infrared data collection and curve-fitting process, the percentages of secondary structure components (a-helix, β-sheet, turn, random coil and β-antiparallel) were obtained. It showed structural diversity among differentFusarium species and variations caused by growth cultures and bodies. The method provides a rapidly quantitative technique to study cell structure of fungi.
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页码：169 / 171
页数：2
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