Neurotrophin growth factors and their receptors as promising blood biomarkers for Alzheimer's Disease: a gene expression analysis study

被引：3

作者：

Asadi, Mohammad Reza ^{[1
,2
]}

Gharesouran, Jalal ^{[1
,2
]}

Sabaie, Hani ^{[1
,2
]}

Zaboli Mahdiabadi, Morteza ^{[3
]}

Mazhari, Seyed Amirhossein ^{[4
]}

Sharifi-Bonab, Mirmohsen ^{[1
]}

Shirvani-Farsani, Zeinab ^{[5
]}

Taheri, Mohammad ^{[6
,7
]}

Sayad, Arezou ^{[8
]}

Rezazadeh, Maryam ^{[1
,2
]}

机构：

[1] Tabriz Univ Med Sci, Clin Res Dev Unit Tabriz Valiasr Hosp, Tabriz, Iran

[2] Tabriz Univ Med Sci, Fac Med, Dept Med Genet, Tabriz, Iran

[3] Shahid Sadoughi Univ Med Sci, Student Res Comm, Yazd, Iran

[4] Azerbaijan Med Univ, Student Res Comm, Baku, Azerbaijan

[5] Shahid Beheshti Univ, Fac Life Sci & Technol, Dept Cell & Mol Biol, Tehran, Iran

[6] Jena Univ Hosp, Inst Human Genet, Jena, Germany

[7] Shahid Beheshti Univ Med Sci, Urol & Nephrol Res Ctr, Tehran, Iran

[8] Shahid Beheshti Univ Med Sci, Loghman Hakim Hosp, Dept Med Genet, Tehran, Iran

来源：

MOLECULAR BIOLOGY REPORTS | 2024年 / 51卷 / 01期

关键词：

Alzheimer's Disease; Neurotrophin growth factors; Biomarkers; Peripheral blood; Gene expression; Bayesian regression; ROC curve analysis; MILD COGNITIVE IMPAIRMENT; CHOLINERGIC NEURONS; NERVOUS-SYSTEM; TRK RECEPTORS; TAU-PROTEIN; BRAIN; BDNF; DEMENTIA; BETA; PROGRESSION;

D O I：

10.1007/s11033-023-08959-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background Alzheimer's disease (AD) is a multifaceted neurological ailment affecting more than 50 million individuals globally, distinguished by a deterioration in memory and cognitive abilities. Investigating neurotrophin growth factors could offer significant contributions to understanding AD progression and prospective therapeutic interventions.Methods and results The present investigation collected blood samples from 50 patients diagnosed with AD and 50 healthy individuals serving as controls. The mRNA expression levels of neurotrophin growth factors and their receptors were measured using quantitative PCR. A Bayesian regression model was used in the research to assess the relationship between gene expression levels and demographic characteristics such as age and gender. The correlations between variables were analyzed using Spearman correlation coefficients, and the diagnostic potential was assessed using a Receiver Operating Characteristic curve. NTRK2, TrkA, TrkC, and BDNF expression levels were found to be considerably lower (p-value < 0.05) in the blood samples of AD patients compared to the control group. The expression of BDNF exhibited the most substantial decrease in comparison to other neurotroph in growth factors. Correlation analysis indicates a statistically significant positive association between the genes. The ROC analysis showed that BDNF exhibited the greatest Area Under the Curve (AUC) value of 0.76, accompanied by a sensitivity of 70% and specificity of 66%. TrkC, TrkA, and NTRK2 demonstrated considerable diagnostic potential in distinguishing between cases and controls.Conclusion The observed decrease in the expression levels of NTRK2, TrkA, TrkC, and BDNF in AD patients, along with the identified associations between specific genes and their diagnostic capacity, indicate that these expressions have the potential to function as biomarkers for the diagnosis and treatment of AD.

引用

页数：11

共 65 条

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