Interactions between genotypes and culture media components for improved in vitro response of rye (Secale cereale L.) inbred lines

被引：25

作者：

Popelka J.C. ^{[1
]}

Altpeter F. ^{[1
]}

机构：

[1] I. Pflanz. Kulturpflan. Gatersleben, AG Gentransfer, 06400 Gatersleben

来源：

Plant Cell Reports | 2001年 / 20卷 / 07期

关键词：

Genotype-culture medium interactions; In vitro system; Inbred lines; Rye (Secale cereale L.); Tissue culture;

D O I：

10.1007/s002990100369

中图分类号：

学科分类号：

摘要：

Rye is one of the most recalcitrant species in tissue culture. A multi-factorial experimental design allowed the development of genotype-specific tissue culture protocols, maximising the plant regeneration response from rye tissue culture. Precocious germination of explants, callus induction and callus maintenance (formation of rhizogenic and caulogenic callus) as well as regeneration response (percentage of regenerating calli and number of regenerated plants per callus) were significantly influenced by genotype, carbohydrate and auxin sources and their respective concentrations and interactions. Genotypes differed in the callus response to media sterilisation procedure, basic salt composition, gelling agent, CuSO4 complementation and illumination. These genotype-specific culture protocols will enable the development of efficient genetic transformation protocols for rye.

引用

页码：575 / 582

页数：7

共 24 条

[1]

Altpeter F., Posselt U.K., Improved plant regeneration from cell suspensions of commercial cultivars, breeding and inbred lines of perennial ryegrass (Lolium perenne L.), J Plant Physiol, 156, pp. 790-796, (2000)

[2]

Barro F., Cannell M.E., Lazzeri P.A., Barcelo P., The influence of auxins on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants, Theor Appl Genet, 97, pp. 684-695, (1998)

[3]

Barro F., Martin A., Lazzeri P.A., Barcelo P., Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum, Euphytica, 108, pp. 161-167, (1999)

[4]

Carman J.G., Jefferson N.E., Campbell W.F., Induction of embryogenic Triticum aestivum L. calli. I. Quantification of cultivar and culture medium effects, Plant Cell Tissue Organ Cult, 10, pp. 101-113, (1987)

[5]

Carman J.G., Jefferson N.E., Campbell W.F., Induction of embryogenic Triticum aestivum L. calli. II. Quantification of addenda and other culture variable effects, Plant Cell Tissue Organ Cult, 10, pp. 115-128, (1987)

[6]

Dahleen L.S., Improved plant regeneration from barley callus cultures by increased copper levels, Plant Cell Tissue Organ Cult, 43, pp. 267-269, (1995)

[7]

Daniel G., Anther culture in rye: Improved plant regeneration using modified MS-media, Plant Breed, 110, pp. 259-261, (1993)

[8]

Eapen S., Rao P.S., Callus induction and plant regeneration from immature embryos of rye and triticale, Plant Cell Tissue Organ Cult, 1, pp. 221-227, (1982)

[9]

Eapen S., Rao P.S., Plant regeneration from immature inflorescence callus cultures of wheat, rye and triticale, Euphytica, 34, pp. 153-159, (1985)

[10]

Flehinghaus T., Deimling S., Geiger H.H., Methodical improvements in rye anther culture, Plant Cell Rep, 10, pp. 397-400, (1991)

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