The effect of oxidized glutathione and its pharmacological analogue glutoxim on intracellular Ca2+ concentration in macrophages Ca2+

被引：0
作者：
Kurilova L.S. [1 ]
Krutetskaya Z.I. [1 ]
Lebedev O.E. [1 ]
Antonov V.G. [1 ]
机构：
[1] Department of Biophysics, St. Petersburg State University, St. Petersburg
来源：
Cell and Tissue Biology | 2008年 / 2卷 / 3期
关键词：
Glutoxim; Oxidized glutathione; Peritoneal macrophages; Tyrosine kinases; Tyrosine phosphatases;
D O I：
10.1134/S1990519X08030139
中图分类号：
学科分类号：
摘要：
Using Fura-2AM microfluorimetry, the effect of oxidized glutathione (GSSG) and its pharmacological analogue glutoxim on the intracellular Ca2+ concentration in rat peritoneal macrophages was investigated. It was shown that GSSG or glutoxim increase the intracellular Ca2+ concentration by inducing Ca2+ mobilization from thapsigargin-sensitive Ca2+ stores and subsequent Ca2+ entry from external medium. Dithiothreitol, which reduces S-S-bonds in proteins, completely prevents or reverses the increase of intracellular Ca2+ concentration induced by GSSG or glutoxim. This suggests that the increase of intracellular Ca2+ concentration induced by GSSG or glutoxim can be mediated by their interactions with functionally important SH-groups of proteins involved in Ca2+-signaling. Two structurally different tyrosine kinase inhibitors genistein and methyl-2,5-dihydroxycinnamate prevent or completely reverse the increase in the intracellular Ca2+ concentration induced by GSSG or glutoxim. On the contrary, tyrosine phosphatase inhibitor Na orthovanadate enhances the increase of intracellular Ca2+ concentration evoked by oxidizing agents. The data suggest that tyrosine kinases and tyrosine phosphatases are involved in the regulatory effect of GSSG and glutoxim on the intracellular Ca2+ concentration in macrophages. © MAIK Nauka 2008.
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页码：322 / 332
页数：10
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