Hyaluronic acid production and characterization by novel Bacillus subtilis harboring truncated Hyaluronan Synthase

被引：0
作者：
Fatemeh Sadat Amjad Zanjani
Shadi Afrasiabi
Dariush Norouzian
Gholamreza Ahmadian
Sara Ali Hosseinzadeh
Alireza Fayazi Barjin
Reza Ahangari Cohan
Malihe Keramati
机构：
[1] Pasteur Institute of Iran,Department of Nanobiotechnology, New Technologies Research Group
[2] National Institute for Genetic Engineering and Biotechnology (NIGEB),Department of Industrial and Environmental Biotechnology
来源：
AMB Express | / 12卷
关键词：
Hyaluronic acid; Hyaluronan synthase; Transmembrane domain; Truncation; Molecular weight;
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摘要：
Hyaluronic Acid (HA) is a natural biopolymer that has important physiological and industrial applications due to its viscoelastic and hydrophilic characteristics. The responsible enzyme for HA production is Hyaluronan synthase (HAS). Although in vitro structure–function of intact HAS enzyme has been partly identified, there is no data on in vivo function of truncated HAS forms. In the current study, novel recombinant Bacillus subtilis strains harboring full length (RBSFA) and truncated forms of SeHAS (RBSTr4 and RBSTr3) were developed and HA production was studied in terms of titer, production rate and molecular weight (Mw). The maximum HA titer for RBSFA, RBSTr4 and RBSTr3 was 602 ± 16.6, 503 ± 19.4 and 728 ± 22.9 mg/L, respectively. Also, the HA production rate was 20.02, 15.90 and 24.42 mg/L.h−1, respectively. The findings revealed that RBSTr3 produced 121% and 137% more HA rather than RBSFA and RBSTr4, respectively. More interestingly, the HA Mw was about 60 kDa for all strains which is much smaller than those obtained in prior studies.
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