Cloning, sequencing and characterization of the genome of a recombinant norovirus of the rare genotype GII.P7/GII.6 in China

被引：0
作者：
Hui Cai
Yongxin Yu
Miao Jin
Yingjie Pan
Shuling Yan
Yongjie Wang
机构：
[1] Shanghai Ocean University,College of Food Science and Technology
[2] Ministry of Agriculture,Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai)
[3] Chinese Center for Disease Control and Prevention,National Institute for Viral Disease Control and Prevention
[4] University of Göttingen,Institute of Biochemistry and Molecular Cell Biology
来源：
Archives of Virology | 2017年 / 162卷
关键词：
Capsid Protein; Recombinant Strain; Recombination Breakpoint; Amino Acid Multiple Sequence Alignment; SimPlot Analysis;
D O I：
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中图分类号：
学科分类号：
摘要：
The genome sequence of a rare recombinant norovirus (NoV) genotype obtained from clinical samples in China was determined using one-step reverse transcription PCR. It was identified as the GII.P7/GII.6 genotype using both phylogenetic and SimPlot analyses. A high degree of variability was observed in the P2 subdomain, especially in the B-loop structure. The recombination breakpoints of all available GII.P7/GII.6 strains were mapped to two different positions within the RdRp region, both of which were at least 40 nt upstream of the overlap of ORF1 and 2. The GII.P7/GII.6 genotype appears to have been circulating in Asia for at least 10 years.
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页码：2053 / 2059
页数：6
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