Effect of Extraneous Zinc on Calf Intestinal Alkaline Phosphatase

被引：0

作者：

ShuLian Yan

YongLi Liu

XiaoJuan Tian

YingXia Zhang

HaiMeng Zhou

机构：

[1] Capital University of Medical Science,Department of Chemistry

[2] Tsinghua University,Department of Biological Science and Biotechnology

[3] Tsinghua University,Protein Science Laboratory of the Ministry of Education, School of Life Science and Engineering

来源：

Journal of Protein Chemistry | 2003年 / 22卷

关键词：

Alkaline phosphatase; zinc ion; inhibition; activation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The effect of extraneous zinc on calf intestinal alkaline phosphatase was studied for quick reversible binding and slow irreversible binding of zinc ions at various concentrations. Under the conditions of slow binding of zinc to CIP increasing Zn2+ (less than 1.0 mM, nM/nE 1.0 × 106) inhibited enzymatic activity, and further increasing Zn2+ resulted in an increase of activity. For quick reversible binding of Zn2+, the effect on CIP activity changed at lower concentrations of substrate, indicating a complex cooperativity between Zn2+ and pNPP. Both protein intrinsic emission fluorescence and ANS-bound protein fluorescence, as well as circular dichroism spectra have shown that the binding of zinc ions changed the enzyme conformation, which was the reason for the changes in enzyme activity induced by extraneous zinc.

引用

页码：371 / 375

页数：4