Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

被引：0
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作者：
Xiang Ji
Xiaomin Si
Yi Zhang
Huawei Zhang
Feng Zhang
Caixia Gao
机构：
[1] Chinese Academy of Sciences,State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology
[2] University of Chinese Academy of Sciences,Key Laboratory of Plant Stress Research, College of Life Science
[3] Shandong Normal University,Department of Plant and Microbial Biology
[4] University of Minnesota,Center for Precision Plant Genomics
[5] University of Minnesota,undefined
来源：
Genome Biology | / 19卷
关键词：
CRISPR/Cas9 system; Geminivirus; Off-target; Virus-inducible; Virus-resistance;
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摘要：
The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
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