Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

被引:0
作者
Kayla Carey
Junghyun Ryu
Kyungjun Uh
Andrea J. Lengi
Sherrie Clark-Deener
Benjamin A. Corl
Kiho Lee
机构
[1] Virginia Tech,Department of Animal and Poultry Sciences
[2] Virginia Tech,Department of Dairy Science
[3] Virginia Tech,Large Animal Clinical Sciences
来源
BMC Biotechnology | / 19卷
关键词
CRISPR/Cas9; Off-targeting; Genome-editing; Embryo development;
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[1]  
Carlson DF(2012)Efficient TALEN-mediated gene knockout in livestock Proc Natl Acad Sci U S A 109 17382-17387
[2]  
Tan W(2011)Generation of PPARgamma mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning Cell Res 21 979-982
[3]  
Lillico SG(2013)Multiplex Genome Engineering Using CRISPR/Cas Systems Science (New York, NY) 339 819-823
[4]  
Stverakova D(2013)ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering Trends in Biotechnology 31 397-405
[5]  
Proudfoot C(2013)High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells Nat Biotechnol 31 822-826
[6]  
Christian M(2017)Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs Transgenic Res 26 97-107
[7]  
Voytas DF(2018)Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation PLoS One 13 338-346
[8]  
Long CR(2016)Efficient production of biallelic GGTA1 knockout pigs by cytoplasmic microinjection of CRISPR/Cas9 into zygotes Xenotransplantation 23 970-978
[9]  
Whitelaw CB(2016)Generation of RUNX3 knockout pigs using CRISPR/Cas9-mediated gene targeting Reprod Domest Anim 51 445-452
[10]  
Fahrenkrug SC(2017)Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting J Biomed Res 31 184-191