Micropropagation of endemic Corynandra chelidonii var. pallae (Cleomaceae) through nodal explants and validation of their genetic integrity by ISSR markers

被引：0
作者：
Sirangi S. [1 ]
Jogam P. [2 ]
Rohela G.K. [3 ]
Ragan A. [2 ]
Raju V.S. [1 ]
机构：
[1] Plant Systematics Laboratory, Department of Botany, Kakatiya University, Telangana, Warangal
[2] Department of Biotechnology, Kakatiya University, Telangana, Warangal
[3] Biotechnology Section, Central Sericultural Research and Training Institute, Central Silk Board, Pampore, Jammu and Kashmir
来源：
Vegetos | 2022年 / 35卷 / 2期
关键词：
Corynandra chelidonii var. pallae; Genetic fidelity analysis; ISSR; Organogenesis; Plant regeneration;
D O I：
10.1007/s42535-021-00302-1
中图分类号：
学科分类号：
摘要：
Micropropagation through nodal explants was attempted for a rare and endemic taxon Corynandra chelidonii var. pallae (Cleomaceae) in view of its medicinal uses, bioactive compounds, declining natural populations due to intermittent sterility, low seed set and erratic seed germination. Multiple shoots were regenerated directly from the nodal explants on Murashige and Skoog’s medium containing different concentrations and combinations of cytokinins and auxins. High-frequency multiple shoots of 8.15 ± 0.249 with 1.63 ± 0.031 shoot length (cm) were obtained on MS medium augmented with the combination of 0.5 mg/L of thidiazuron and 1.0 mg/L of indole-3-acetic acid. Directly regenerated shoots were rooted with 95% root induction on a half-strength MS medium supplemented with 1.0 mg/L of indole-3 butyric acid. The in vitro raised plantlets were hardened in plastic pots and acclimatized initially in the greenhouse conditions and later transferred to the field conditions with a 78% of survival rate. The genetic fidelity of in vitro generated and field transferred plantlets with that of the mother plant was checked by carrying ISSR marker-based polymerase chain reaction method. This protocol on direct organogenesis and genetic fidelity analysis in Corynadra chellidonii var. pallae can be successfully employed for large-scale multiplication and conservation. © 2021, Society for Plant Research.
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页码：511 / 519
页数：8
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