The Production of Reactive Oxygen and Halogen Species by Neutrophils in Response to Monomeric Forms of Myeloperoxidase

被引：1

作者：

Gorudko I.V. ^{[1
]}

Mikhalchik E.V. ^{[2
]}

Sokolov A.V. ^{[2
,3
,4
,5
]}

Grigorieva D.V. ^{[1
]}

Kostevich V.A. ^{[2
,3
]}

Vasilyev V.B. ^{[3
,4
]}

Cherenkevich S.N. ^{[1
]}

Panasenko O.M. ^{[2
]}

机构：

[1] Belarusian State University, Minsk

[2] Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Moscow

[3] Institute of Experimental Medicine, St. Petersburg

[4] St. Petersburg State University, St. Petersburg

[5] Center of Preclinical Translational Research, Almazov National Medical Research Center, St. Petersburg

来源：

Biophysics | 2017年 / 62卷 / 6期

基金：

俄罗斯科学基金会; 俄罗斯基础研究基金会;

关键词：

halogenative stress; hemi-myeloperoxidase; myeloperoxidase; neutrophils; reactive halogen species; reactive oxygen species;

D O I：

10.1134/S0006350917060069

中图分类号：

学科分类号：

摘要：

It was shown for the first time that myeloperoxidase, a homodimer that consists of two disulfidebonded identical protomers and catalyzes the formation of hypochlorous acid (HOCl), is decomposed by HOCl into monomers (MPO-Cl). Dimeric myeloperoxidase can also be converted into monomers (hemimyeloperoxidase) by reduction of the disulfide bond. In this study, the effects of two monomeric forms of myeloperoxidase, MPO-Cl and hemi-myeloperoxidase, and native dimeric myeloperoxidase on the production of reactive oxygen (•O2 − and H2O2) and halogen (HOCl) species by neutrophils were compared. Neutrophil production of these species was monitored after addition of hemi-myeloperoxidase, MPO-Cl, or dimeric myeloperoxidase and also after the subsequent addition of activators, phorbol-12-myristate-13-acetate or N-formyl-Met-Leu-Phe. HOCl production was assessed by chemiluminescence in the presence of luminol; •O2 − production was assessed by chemiluminescence in the presence of lucigenin and by cytochrome c reduction determined spectrophotometrically, and H2O2 production was measured using fluorimetry with scopoletin. The results indicate that MPO-Cl and hemi-myeloperoxidase, which can occur in blood under halogenative stress, do not prime neutrophil NADPH oxidase, and do not enhance the production of reactive oxygen (•O2 − and H2O2) and halogen (HOCl) species. © 2017, Pleiades Publishing, Inc.

引用

页码：919 / 925

页数：6

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