A novel and rapid method for fatty acid preparation by the lipase-catalyzed hydrolysis of Phoenix tree seeds

Fatty acids are the precursors for the production of fuels, oleochemicals and special health care products. In this study, a novel rapid method for fatty acid (FA) preparation by the enzymatic hydrolysis of Phoenix tree seed, an undeveloped woody oil seed, was developed. High-temperature GC with flame ionization detector (FID) and the hydrolysis ratio were used to monitor reaction progress. Enzyme screening and the effect of reaction variables on the hydrolysis of seeds were evaluated and optimized by response surface methodology. The results showed that among the tested enzymes, Lipozyme TLIM showed the greatest amount of hydrolysis of Phoenix tree seed. FAs can be rapidly prepared by one-step hydrolysis of Phoenix tree seeds using Lipozyme TLIM as the biocatalyst. Under the optimized conditions (6% enzyme load, 1:8 mass ratio of seed to water, 47.7 °C and 16 min), the maximum hydrolysis ratio (96.4 ± 1.1%) can be achieved. The effect of reaction variables on the hydrolysis decreased in the following order: reaction time > enzyme load > substrate ratio of seed to water > reaction temperature. This work provides a novel and rapid method for FA preparation from oil seeds.