Determination of the Amino Acid Sequence of a New Phospholipase A2 (MIDCA1) Isolated from Micrurus dumerilii carinicauda Venom

被引：0
作者：
Cháriston A. Dal Belo
Marcos H. Toyama
Daniela de O. Toyama
Sergio Marangoni
F. B. Moreno
Benildo S. Cavada
Marcos D. Fontana
S. Hyslop
E. M. Carneiro
Antonio C. Boschero
机构：
[1] State University of Campinas (UNICAMP),Department of Pharmacology, School of Medical Sciences
[2] State University of Campinas,Department of Physiology and Biophysics, Institute of Biology
[3] (UNICAMP),Department of Biochemistry, Institute of Biology
[4] State University of Campinas,Department of Biochemistry and Molecular Biology
[5] (UNICAMP),undefined
[6] Federal University of Ceará (UFC),undefined
来源：
The Protein Journal | 2005年 / 24卷
关键词：
Amino acid sequence; neurotoxic and myotoxic; phospholipase A;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new Phospholipase A2 (PLA2) from Micrurus dumerilii carinicauda venom was isolated and its primary structure determined. This new PLA2 showed a low enzymatic activity when compared with other PLA2s and it is moderately basic with an isoelectric point of 8.0. Its amino acid sequence showed the presence of 120 amino acid residues and its sequence was: NLIQFLNMIQCTTPGREPLVAFANYGCYCGRGGSGTPVDELDRCCQVHDNCYDTAKKVFGCSPYFTMYSYDCSEGKLTCKDNNTKCKAAVCNCDRTAALCFAKAPYNDKNYKIDLTKRCQ. The structural model of MIDCA1, when compared with other strong neurotoxic PLA2s, such as Naja naja, showed significant differences in the β-wing and neurotoxic sites, despite the high level of amino acid sequence similarity. These observations indicate a dissociation between the biological and catalytic activity of this new PLA2, supporting the view that other regions of the protein are involved in the biological effects.
引用
收藏
页码：147 / 153
页数：6
相关论文
共 74 条
  • [1] Alape-Giron A.(1996)undefined FEBS Lett. 380 29-32
  • [2] Persson B.(1996)undefined Electrophoresis 17 443-453
  • [3] Cedelund E.(1991)undefined Eur. J. Biochem. 202 493-500
  • [4] Flores-Diaz M.(1989)undefined J. Biol. Chem. 264 13289-13297
  • [5] Gutierrez J. M.(1990a)undefined Biochim. Biophys. Acta 1037 7-15
  • [6] Thelestam M.(1990b)undefined J. Mol. Evol. 31 228-238
  • [7] Bergman T.(1994.)undefined J. Biol. Chem. 269 13057-13060
  • [8] Joernavall H.(1997)undefined Toxicon 35 1193-1203
  • [9] Anderson N. G.(1976)undefined J. Biol. Chem. 251 7343-7347
  • [10] Anderson N. L.(1980)undefined Eur. J. Biochem. 112 493-499
12345678