The Expression of Cytokine Genes in the Peritoneal Macrophages and Splenic CD4- and CD8-Positive Lymphocytes of the Nonobese Diabetic Mice

被引：0
作者：
Nik-Soriani Yaacob
Mohd-Arifin Kaderi
Mohd-Nor Norazmi
机构：
[1] Universiti Sains Malaysia,Department of Chemical Pathology, School of Medical Sciences
[2] Kubang Kerian,undefined
[3] School of Health Sciences,undefined
[4] Universiti Sains Malaysia,undefined
来源：
Journal of Clinical Immunology | 2004年 / 24卷
关键词：
Cytokines; multiplex PCR; NOD mouse strain; type 1 diabetes;
D O I：
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学科分类号：
摘要：
Type 1 diabetes is an autoimmune disease that results from the destruction of the insulin-producing pancreatic β islet cells, probably via the influence of cytokines. However, direct correlation between the expression of selected cytokines by various immune cells at different time points during the progression of the disease has not yet been clearly demonstrated. In this study, we showed that the mRNA expression of the pro-inflammatory cytokines, TNF-α, IL-1β, IL-6, and GM-CSF, were increased while the anti-inflammatory cytokine, TGF-β, decreased in the peritoneal macrophages of nonobese diabetic (NOD) mice. IL-6 expression however decreased when the mice became diabetic. Surprisingly the expression of IFN-γ and IL-2 by splenic CD4+ cells were lower in 5-week-old NOD mice as compared to the nonobese diabetic resistant (NOR) control mice, but their expression was higher in older NOD mice. The expression of IL-4 and IL-10 decreased in splenic CD4-positive lymphocytes. Splenic CD8-positive lymphocytes expressed increased levels of IFN-γ and IL-10 but the latter decreased sharply when diabetes occurred. The relevance of these findings to the pathogenesis of type 1 diabetes is discussed.
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页码：177 / 184
页数：7
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  • [21] Discolo G(1994)Local production of human IL-6 promotes insulitis but retards the onset of insulin-dependent diabetes mellitus in nonobese diabetic mice Int Immunol 6 1829-1837
  • [22] Yasukawa K(1997)Interleukin (IL)-6 directs the differentiation of IL-4-producing CD4+ T cells J Exp Med 185 461-470
  • [23] Hammacher A(1991)Granulocyte-macrophage colony-stimulating factor promotes the proliferation of human alveolar macrophages in vitro J Immunol 147 1266-1272
  • [24] Moritz RL(1999)Tumor necrosis factor-alpha: A continuum of liability between insulin-dependent diabetes mellitus, non-insulin-dependent diabetes mellitus and carcinoma Med Hypotheses 52 319-323
  • [25] Simpson RJ(2003)IL-1β, IFN-β and TNF-α increase vulnerability of pancreatic β cells to autoimmune destruction J Autoimmun 20 303-312
  • [26] McDuffie M(1996)Local expression of transgene encoded TNF α in islets prevents autoimmune diabetes in nonobese diabetic (NOD) mice by preventing the development of auto-reactive islet-specific T cells J Exp Med 184 1963-1974
  • [27] Kikutani H(1997)Chronic tumor necrosis factor alters T cell responses by attenuating T cell receptor signaling J Exp Med 185 1573-1584
  • [28] Makino S(2001)A dual role of TNF-α in the type-1 diabetes: Islet-specific expression abrogates the ongoing autoimmune process when induced late but not early during pathogenesis J Immunol 166 7023-7032
  • [29] Delovitch TL(2000)Abrogation of TGF-beta signaling in T cells leads to spontaneous T cell differentiation and autoimmune disease Immunity 12 171-181
  • [30] Singh B(1998)TGF-β 1 alters APC preference, polarizing islet antigen responses toward a Th2 phenotype Immunity 8 601-613
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