Genotypic variation in anther culture and effect of ovary coculture in durum wheat

被引：0
作者：
F. J'Aiti
O. Benlhabib
H. C. Sharma
S. El Jaafari
I. El Hadrami
机构：
[1] University Cadi Ayyad,Laboratory of Plant Physiology, Department of Biology
[2] Faculty of Sciences Semlalia,Laboratory of Tissues Culture and Cytogenetic, Department of Agronomy
[3] Rabat-Institutes,Department of Agronomy
[4] Purdue University,undefined
[5] Laboratoire de Biotechnologie et Amélioration des Plantes,undefined
[6] Faculté des Sciences,undefined
来源：
Plant Cell, Tissue and Organ Culture | 1999年 / 59卷
关键词：
androgenic potential; anther culture; anther-ovary coculture; durum wheat;
D O I：
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中图分类号：
学科分类号：
摘要：
The response of anthers to in vitro culture and the effect of coculture of ovaries on anther culturability have been studied in responsive and recalcitrant cultivars of durum wheat (Triticum turgidum ssp. durum) from Morocco and ICARDA. A large genotypic-dependence of anther culture has been shown in 18 cultivars. Their response in term of callus and embryo induction varied from 0 to 13%. Coculture of ovaries with anthers enhanced the response of the most responsive genotype (cv. Sarif) and removed the recalcitrance in Cocorit and Isly cultivars. However, there was no effect of anther-ovary coculture on green plant regeneration. The implication of the genome and the media conditioning by the ovaries on anther response is discussed.
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页码：71 / 76
页数：5
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