Optimization of RNA isolation from brittle leaf disease affected date palm leaves and construction of a subtractive cDNA library

被引：10

作者：

Saïdi M.N. ^{[1
]}

Gargouri-Bouzid R. ^{[1
]}

Rayanni M. ^{[1
]}

Drira N. ^{[2
]}

机构：

[1] Laboratoire des Biotechnologies Vegetales Appliquees a l'Amelioration des Cultures, Ecole Nationale d'Ingénieurs de Sfax, Sfax 3038

[2] Faculté des Sciences de Sfax, Sfax 3018

来源：

Molecular Biotechnology | 2009年 / 41卷 / 1期

关键词：

Date palm leaves; Phenolic compounds; RNA isolation; Subtractive hybridization;

D O I：

10.1007/s12033-008-9104-1

中图分类号：

学科分类号：

摘要：

A simple and efficient method was described here for the isolation of high-quality RNA from date palm leaves affected with Brittle Leaf Disease (BLD) and containing high amount of phenolic compounds. The procedure was based on the use of a non-ionic detergent Nonidet-P40 (NP-40), Polyvinylpyrrolidone (PVP), and β-mercaptoethanol in the extraction buffer in order to isolate cytoplasmic RNA and to prevent the oxidation of phenolic compounds. This method allowed the isolation of intact RNA, suitable for cDNA synthesis and library construction. Differential screening of the subtractive cDNA library from affected leaf RNA led to the identification of some BLD-induced genes. © 2008 Humana Press.

引用

页码：63 / 68

页数：5

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