Olfactory Ensheathing Cell-Conditioned Medium Protects Astrocytes Exposed to Hydrogen Peroxide Stress

被引：0
作者：
Liu Jinbo
Liu Zhiyuan
Zhang Zhijian
Ding WenGe
机构：
[1] The Third Affiliated Hospital of Suzhou University,Department of Orthopedics
[2] School of Medicine,undefined
[3] Jiangsu University,undefined
来源：
Cellular and Molecular Neurobiology | 2013年 / 33卷
关键词：
Olfactory ensheathing cell; Astrocytes; H; O; Apoptosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The purpose of this study was to observe the effects of olfactory ensheathing cell conditioned medium (OECCM) on damaged astrocytes after exposure to H2O2 in vitro. OECCM was used to treat astrocytes after injury, which was induced by exposure to 500 μmol/L H2O2 for 20 min. The cell morphology was then observed under a light microscope, cell viability assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell ultrastructure observed with transmission electron microscopy (TEM), and apoptosis assessed by Annexin V staining followed by cytometry and Western blot. H2O2 induced severe damage to astrocytes as evidenced by decreased cell number, pathological changes in cell morphology, and significantly elevated cell apoptosis. Cells incubated with OECCM displayed significantly improved cell viability and decreased cell apoptotic rate. Under TEM, H2O2-treated cells showed partially broken plasma membranes, swollen rough endoplasmic reticula, visible vacuoles, and swollen or deformed mitochondria with ruptured cristae. Incubation with OECCM significantly ameliorated these pathological changes in astrocytes. These results suggest that OECCM may protect astrocytes from oxidative damage by promoting cell survival while reducing apoptosis of the damaged cells.
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页码：699 / 705
页数：6
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