Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete chrysosporium

被引：8

作者：

Zhou X. ^{[1
]}

Wen X. ^{[1
]}

Feng Y. ^{[1
]}

机构：

[1] State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University

来源：

Frontiers of Environmental Science & Engineering in China | 2007年 / 1卷 / 1期

关键词：

Fed-batch culture; Glucose feeding; Ligninolytic enzymes; White-rot fungus;

D O I：

10.1007/s11783-007-0017-1

中图分类号：

学科分类号：

摘要：

The present work studied the influence of glucose feeding on the ligninolytic enzyme production of Phanerochaete chrysosporium in a nitrogen-limited (C/N ratio is 56/8.8 mmol/L) medium. Several sets of shaking flask experiments were conducted. The results showed that 2 g/L glucose feeding on the first day of the culture (24 h after the inoculation) simulated both fungal biomass growth and enzyme production. The manganese peroxidase (MnP) activity was 2.5 times greater than that produced in cultures without glucose feeding. Furthermore, the glucose feeding mode in fed-batch culture was also investigated. Compared to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (final concentration) every 24 h produced more enzymes. The peak and total yield of MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively, and the enzyme was kept stable for 4 days with an activity of over 200 U/L. © Higher Education Press 2007.

引用

页码：89 / 94

页数：5

共 10 条

[1]

Li H.R., Biology and Biotechnology of White Rot Fungi, (2005)

[2]

Dosoretz C.G., Chen H.C., Grethlein H.E., Effect of environmental conditions on extracellular protease activity in ligninolytic cultures of Phanerochaete chrysosporium, Appl Environ Microbiol, 56, 2, pp. 395-400, (1990)

[3]

Moreia M.T., Torrado A., Feijoo G., Et al., Manganese peroxidae production by Bjerkandera sp. BOS55, operation in stireed tank reactors, Bioprocess Engineering, 23, pp. 663-667, (2000)

[4]

Galhaup C., Haltrich D., Enhanced formation of laccase activity by the white-rot fungus Trametes pubescens in the presence of copper, Appl Microbiol Biotechnol, 56, pp. 225-232, (2001)

[5]

Yu G.C., Wen X.H., Qian Y., Production of the ligninolytic enzymes by immobilized Phanerochaete chrysosporium in an air atmosphere, World Journal of Microbiology & Biotechnology, 21, pp. 323-327, (2005)

[6]

Zhang Z.H., Shao H., Zhou X.Y., Et al., Production of ligninolytic enzymes by Phanerochaete chrysosporium in three-phase bubble column bioreactor, Chin J Appl Environ Biol, 9, 3, pp. 288-292, (2003)

[7]

Lu S.Y., Wang S.Y., Li H.Z., Chen J., The effect of proteinase excreted by Phanerochaete chrysosporium on its peroxidase activity, 22, pp. 23-26, (2003)

[8]

Cabaleiro D.R., Rodriguez S., Sanroman A., Longo M.A., Characterisation of deactivating agents and their influence on the stability of manganese-dependent peroxidase from Phanerochaete chrysosporium, Journal of Chemical Technology and Biotechnology, 76, pp. 867-872, (2001)

[9]

Tien M., Kirk T.K., Lignin peroxidase of Phanerochaete chrysosporium, Methods in Enzymology, 161, pp. 238-249, (1988)

[10]

Paszczynski A., Crawford R.L., Huynh V.B., Manganese peroxidase of Phanerochaete chrysosporium: Purification, Method Enzymol, 161, pp. 264-270, (1988)

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