A rapid colony screening method for the detection of arsenate-reducing bacteria

被引：0
作者：
S. M. Mandal
K. C. Mondal
S. Dey
B. R. Pati
机构：
[1] Vidyasagar University,Department of Microbiology
[2] IIT Kharagpur,Department of Biotechnology
来源：
Indian Journal of Microbiology | 2007年 / 47卷 / 2期
关键词：
Arsenate-reducing bacteria; Arsenite; Starch-iodine complex;
D O I：
10.1007/s12088-007-0032-8
中图分类号：
学科分类号：
摘要：
A rapid and simple method has been developed for the detection of arsenate reducing bacteria based on the presence of arsenite [As (III)], the end product of anaerobic arsenate [As (V)] respiration. Confirmation of As (III) product is made by the reduction of starch-iodine complex. The method can be used over a large pH range (5.5–9.0) and can easily be determined at arsenite concentration as low as 0.025 mM. Major advantages of this technique are that a large number of samples can be analyzed easily at a time.
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页码：167 / 169
页数：2
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