Effects of pulsed electric fields on DNA of human lymphocytes

被引：0
作者：
J. Delimaris
S. Tsilimigaki
N. Messini-Nicolaki
E. Ziros
S. M. Piperakis
机构：
[1] National Center for Scientific Research “Demokritos”,DNA Repair Laboratory, Institute of Biology
[2] University of Athens,Department of Cell Biology, School of Biology
[3] Laboratory of Microbiology,Biology Unit, Department of Pre
[4] 1st I.K.A. Health Department,School Education
[5] University of Thessaly,undefined
来源：
Cell Biology and Toxicology | 2006年 / 22卷
关键词：
comet assay; DNA damage; humans; pulsed electric fields;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The effects of pulsed electric fields of low frequency (50 Hz) on DNA of human lymphocytes were investigated. The influence of additional external factors, such as hydrogen peroxide (H2O2) and γ-irradiation, as well as the repair efficiency in these lymphocytes, was also evaluated. The comet assay, a very sensitive and rapid method for detecting DNA damage at the single cells level was the method used. A significant amount of damage was observed after exposure to the electric fields, compared to the controls. After 2 h incubation at 37°C, a proportion of damage was repaired. H2O2 and γ-irradiation increased the damage to lymphocytes exposed to pulsed electric fields according to the dose used, while the amount of the repair was proportional to the damage.
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页码：409 / 415
页数：6
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