Agrobacterium-mediated transformation of African violet

被引：0
|
作者：
Antonio Mercuri
Laura De Benedetti
Gianluca Burchi
Tito Schiva
机构：
[1] Istituto Sperimentale per la Floricoltura,
来源：
Plant Cell, Tissue and Organ Culture | 2000年 / 60卷
关键词：
African violet; GUS; PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A method for genetic transformation of Saintpaulia ionantha by co-cultivation of in vitro-grown leaves and petioles with Agrobacterium tumefaciens is described. Two bacterial strains, EHA105 and A281 both harbouring the binary plasmid pKIWI105 carrying the genes uidA and nptII, were used in the experiments. Regenerants were not obtained using the disarmed strain EHA105. The oncogenic strain A281 resulted in efficient transient and stable expression of the transferred traits for petiole explants only. After transformation and regeneration, the integration of the transgenes in the plant genome was confirmed by PCR analysis and Southern hybridization.
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页码：39 / 46
页数：7
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