A plant-based expression system for matching cDNA clones and isozymes

被引：0
作者：
P. R. Matthews
F. Gubler
J. V. Jacobsen
机构：
[1] CSIRO Division of Plant Industry,
来源：
Plant Molecular Biology Reporter | 1997年 / 15卷
关键词：
α-amylase cDNAs; cDNA isozyme matching; isoelectric focusing; microparticle bombardment; oat aleurone; transient expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Isozymes of barley α-amylase were matched to cDNAs that encode them using transient expression in oat aleurone layers. Four cDNAs, including two that are previously unpublished, were inserted into oat aleurone cells by microparticle bombardment. The cDNAs were under the control of theAct1 promoter of rice. Expression levels were sufficient for in-gel detection of enzyme activity following isoelectric focusing of aleurone homogenates. The system has also proved useful in characterizing a hybrid β-glucanase gene.
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页码：163 / 169
页数：6
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