A plant-based expression system for matching cDNA clones and isozymes

被引：0
作者：
P. R. Matthews
F. Gubler
J. V. Jacobsen
机构：
[1] CSIRO Division of Plant Industry,
来源：
Plant Molecular Biology Reporter | 1997年 / 15卷
关键词：
α-amylase cDNAs; cDNA isozyme matching; isoelectric focusing; microparticle bombardment; oat aleurone; transient expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Isozymes of barley α-amylase were matched to cDNAs that encode them using transient expression in oat aleurone layers. Four cDNAs, including two that are previously unpublished, were inserted into oat aleurone cells by microparticle bombardment. The cDNAs were under the control of theAct1 promoter of rice. Expression levels were sufficient for in-gel detection of enzyme activity following isoelectric focusing of aleurone homogenates. The system has also proved useful in characterizing a hybrid β-glucanase gene.
引用
收藏
页码：163 / 169
页数：6
相关论文
共 76 条
[1]  
Aoyagi K.(1990)The expression of barley α-amylase genes in Planta 180 333-340
[2]  
Sticher L.(1990) oocytes Plant Cell 2 633-641
[3]  
Wu M.(1967)Nonlegume hemoglobin genes retain organ-specific expression in heterologous transgenic plants Plant Physiol. 42 398-406
[4]  
Jones R.L.(1985)Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers Plant Physiol. 78 192-198
[5]  
Bogusz D.(1993)Control of α-amylase accumulation by gibberellic acid and calcium in barley aleurone layers Anal. Biochem. 210 235-244
[6]  
Llewellyn D.J.(1992)Reduction of background problems in nonradioactive northern and Southern blot analyses enables higher sensitivity than Plant Cell Rep. 11 323-328
[7]  
Craig S.(1990)P-based hybridizations EMBO J. 9 2517-2522
[8]  
Dennis E.S.(1995)Development of the particle inflow gun for DNA delivery to plant cells Transgenic Res. 4 324-33
[9]  
Appleby C.A.(1995)Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues Plant Cell 7 1879-1891
[10]  
Peacock W.J.(1994)Adenine methylation at dam sites increases transient gene expression in plant cells Plant J. 5 593-604
12345678