Heterologous Kluyveromyces lactis β-galactosidase secretion by Saccharomyces cerevisiae super-secreting mutants

被引：0
作者：
M. Becerra
S. Díaz Prado
E. Cerdán
M.I. González Siso
机构：
[1] Campus da Zapateira s/n,Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
β-galactosidase; protein secretion; super-secreting mutants; yeast;
D O I：
暂无
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学科分类号：
摘要：
Several Saccharomyces cerevisiae strains with a super-secreting phenotype have been transformed using a secretion plasmid containing the LAC4 gene and have proven to be effective in the secretion of Kluyveromyceslactis β-galactosidase. The strain CGY1585 (ssc1-1) showed the highest secretion (1.7 EU ml−1) in the culture medium. As far as we know, Kluyveromyceslactis β-galactosidase is the largest sized protein and the only intracellular one among those secreted by these mutants hitherto. The recombinant strains all grew in lactose media.
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页码：33 / 40
页数：7
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