Anti-cariogenic Characteristics of Rubusoside

被引:0
作者
Jeesoo Kim
Thi Thanh Hanh Nguyen
Juhui Jin
Iis Septiana
Gyu-Min Son
Gang-Hee Lee
You-Jin Jung
Dilshad Qureshi
Il Kyoon Mok
Kunal Pal
Soo-Yeon Yang
Seong-Bo Kim
Doman Kim
机构
[1] Seoul National University,Graduate School of International Agricultural Technology and Institutes of Green Bioscience & Technology
[2] National Institute of Technology,Department of Biotechnology and Medical Engineering
[3] Life Ingredient & Material Research Institute,CJ CheilJedang
来源
Biotechnology and Bioprocess Engineering | 2019年 / 24卷
关键词
Anti-cariogenicity; mutansucrase; natural sweetener; rubusoside;
D O I
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中图分类号
学科分类号
摘要
Streptococcus mutans plays an important role in the development of dental caries in humans by synthesizing adhesive insoluble glucans from sucrose by mutansucrase activity. To explore the anti-cariogenic characteristics of rubusoside (Ru), a natural sweetener component in Rubus suavissimus S. Lee (Rosaceae), we investigated the inhibitory effect of Ru against the activity of mutansucrase and the growth of Streptococcus mutans. Ru (50 mM) showed 97% inhibitory activity against 0.1 U/mL mutansucrase of S. mutans with 500 mM sucrose. It showed competitive inhibition with a Ki value of 1.1 ± 0.2 mM and IC50 of 2.3 mM. Its inhibition activity was due to hydrophobic and hydrogen bonding interactions based on molecular docking analysis. Ru inhibited the growth of S. mutans as a bacteriostatic agent, with MIC and MBC values of 6 mM and 8 mM, respectively. In addition, Ru showed synergistic anti-bacterial activity when it was combined with curcumin. Therefore, Ru is a natural anti-cariogenic agent with anti-mutansucrase activity and antimicrobial activity against S. mutans.
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页码:282 / 287
页数:5
相关论文
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