Effects of continuous cucumber cropping and alternative rotations under protected cultivation on soil microbial community diversity

被引:74
作者
Huaiying Yao
Xiaodan Jiao
Fengzhi Wu
机构
[1] Northeast Agricultural University,Department of Horticultural Sciences
[2] Zhejiang University,Department of Resource Sciences
来源
Plant and Soil | 2006年 / 284卷
关键词
Biolog; continuous; plastic-greenhouse; RAPD; rotation; soil microbial community diversity;
D O I
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中图分类号
学科分类号
摘要
The diversity of soil microbial communities as affected by continuous cucumber cropping and alternative rotations under protected cultivation were evaluated using community level physiological profiles (CLPP) and random amplified polymorphic DNA (RAPD) analysis. The soils were selected from six cucumber cropping systems, which cover two cropping practices (rotation and continuous cropping) and a wide spectrum for cucumber cropping history under protected cultivation. Shannon–Weaver index and multivariate analysis were performed to characterize variations in soil microbial communities. Both CLPP and RAPD techniques demonstrated that cropping systems and plastic-greenhouse cultivation could considerably affect soil microbial functional diversity and DNA sequence diversity. The open-field soil had the highest Shannon–Weaver index (3.27 for CLPP and 1.50 for RAPD), whereas the lowest value occurred in the 7-year continuous protected cultivation soil (3.27 for CLPP and 1.50 for RAPD). The results demonstrated that continuous plastic-greenhouse cultivation and management can cause the reduction in the species diversity of the biota. Higher Shannon–Weaver index and coefficients of DNA sequence similarity were found in soils under rotation than those under continuous cropping. Cluster analysis also indicated that microbial community profiles of continuous cultivation soils were different from profiles of rotation soils. The reduction in diversity of microbial communities found in continuous cultivation soils as compared with rotation soils might be due to the differences in the quantity, quality and distribution of soil organic matter.
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页码:195 / 203
页数:8
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