Reduction in C-terminal amidated species of recombinant monoclonal antibodies by genetic modification of CHO cells

被引:0
作者
Mihaela Škulj
Dejan Pezdirec
Dominik Gaser
Marko Kreft
Robert Zorec
机构
[1] Mengeš,Sandoz Biopharmaceuticals
[2] Lek Pharmacetucals d.d,Laboratory of Neuroendocrinology – Molecular Cell Physiology
[3] Celica Biomedical Centre,Biotechnical Faculty
[4] Institute of Pathophysiology,undefined
[5] Faculty of Medicine,undefined
[6] University of Ljubljana,undefined
[7] University of Ljubljana,undefined
来源
BMC Biotechnology | / 14卷
关键词
C-terminal amidation; Genetic modification; Recombinant monoclonal antibody;
D O I
暂无
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 173 条
[1]  
Kim JY(2012)CHO cells in biotechnology for production of recombinant proteins: current state and further potential Appl Microbiol Biotechnol 93 917-930
[2]  
Kim YG(2005)Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals Appl Microbiol Biotechnol 68 283-291
[3]  
Lee GM(1999)Enhancing therapeutic glycoprotein production in Chinese hamster ovary cells by metabolic engineering endogenous gene control with antisense DNA and gene targeting Glycobiology 9 841-850
[4]  
Butler M(2007)Glycosylation of therapeutic proteins in different production systems Acta Paediatr Suppl 96 17-22
[5]  
Warner TG(1995)Processing of C-terminal lysine and arginine residues of proteins isolated from mammalian cell culture J Chromatogr A 705 129-134
[6]  
Werner RG(2013)C-terminal modification of monoclonal antibody drugs: amidated species as a general product-related substance Int J Biol Macromol 52 139-147
[7]  
Kopp K(2011)Characterization of the basic charge variants of a human IgG1: effect of copper concentration in cell culture media MAbs 3 577-583
[8]  
Schlueter M(2007)Cation exchange-HPLC and mass spectrometry reveal C-terminal amidation of an IgG1 heavy chain Anal Biochem 360 75-83
[9]  
Harris RJ(1994)C-terminal amidated peptides: production by the in vitro enzymatic amidation of glycine-extended peptides and the importance of the amide to bioactivity Enzyme Microb Technol 16 450-456
[10]  
Tsubaki M(2001)Structural studies on C-amidated amino acids and peptides: structures of hydrochloride salts of C-amidated Ile, Val, Thr, Ser, Met, Trp, Gln and Arg, and comparison with their C-unamidated counterparts Acta Crystallogr B 57 72-81