Determination of phenolic acids and a flavonoid in Eleusine coracana (L.) by semi-preparative HPLC photo diode array detector

被引：0
|
作者：
Jignasu P. Mehta
Chirag R. Fultariya
Pravin H. Parmar
Sohil H. Vadia
Balubhai A. Golakiya
机构：
[1] Maharaja Krishankumarsinhji Bhavnagar University,Department of Chemistry, DST
[2] Junagadh Agricultural University,FIST, UGC NON
来源：
Journal of Analytical Chemistry | 2015年 / 70卷
关键词：
phenolic compounds; flavonoids; medicinal herbs; semi-preparative HPLC;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Semi preparative HPLC procedure is proposed for the determination of six phenolic acids and one flavonoid in Eleusine coracana (L.) with gradient elution and photodiode array detection. The influence of composition of the mobile phase concentration of the mix modifier and temperature on the separation of gallic acid, p-hydroxybenzoic, vanillic, caffeic, ferulic, and cinnamic acids and quercetin for 90 min is studied. The procedure was applied for the separation of phenolic compounds in methanolic extracts of Eleusine coracana (L.). The lower limits of quantification of phenolic compounds and the flavonoid are 0.050–0.150 μg/mL.
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页码：369 / 373
页数：4
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