Isolation and characterization of 15 polymorphic microsatellite markers for the fig-pollinating wasp, Blastophaga nipponica (Hymenoptera: Agaonidae)

被引：0
作者：
Junko Kusumi
Zhi-Hui Su
机构：
[1] Kyushu University,Department of Environmental Changes, Faculty of Social and Cultural Studies
[2] JT Biohistory Research Hall,Department of Biological Sciences, Graduate School of Science
[3] Osaka University,undefined
来源：
Applied Entomology and Zoology | 2014年 / 49卷
关键词：
Agaonidae; Microsatellites; Inbreeding;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We developed microsatellite markers for the fig-pollinating wasp Blastophaga nipponica Grandi using a dual-suppression-PCR technique. Twenty-one candidates of microsatellite loci were obtained, of which 15 yielded scorable patterns. The degree of polymorphism for the 15 loci was further characterized using summary statistics describing the genetic variation in 60 individuals from three natural populations in Japan. All 15 loci were polymorphic and yielded 2–27 alleles per locus. Overall observed heterozygosity (HO) and expected heterozygosity (HE) were 0.465 and 0.631, respectively. As expected, based on the inbreeding tendency of this species, the mean inbreeding coefficient (FIS) was high (= 0.255). These markers will contribute to studies on the population structure of this species.
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页码：487 / 491
页数：4
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