Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis

被引：0
|
作者：
Kan Yan Chloe Li
Hector Dejea
Koen De Winne
Anne Bonnin
Valentino D’Onofrio
Janneke A. Cox
Patricia Garcia-Canadilla
Martin Lammens
Andrew C. Cook
Bart Bijnens
Amélie Dendooven
机构：
[1] University College London,Institute of Cardiovascular Science
[2] Paul Scherrer Institute,Swiss Light Source
[3] ETH Zurich,Department of Biomedical Engineering
[4] Lund University,Department of Pathology
[5] MAX IV Laboratory,Faculty of Medicine and Life Sciences
[6] Antwerp University Hospital and University of Antwerp,Department of Infectious Diseases and Immunity
[7] Hasselt University, Interdisciplinary Cardiovascular Research Group
[8] Jessa Hospital,BCNatal Barcelona Center for Maternal
[9] Sant Joan de Déu Research Institute (IRSJD),Fetal and Neonatal Medicine
[10] Hospital Sant Joan de Déu and Hospital Clínic,undefined
[11] University of Barcelona,undefined
[12] ICREA,undefined
[13] IDIBAPS,undefined
来源：
Histochemistry and Cell Biology | 2023年 / 160卷
关键词：
X-ray phase contrast imaging; Pathology; Needle biopsies;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.
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页码：377 / 389
页数：12
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