Immunofluorescent Identification of Melanocytes in Murine Hair Follicles

被引：0
作者：
Dheshnie Govender
Lester M. Davids
Susan H. Kidson
机构：
[1] University of Cape Town Medical School,Department of Human Biology, Anatomy Building, Faculty of Health Sciences
来源：
Journal of Molecular Histology | 2006年 / 37卷
关键词：
flourescent microscopy; hydrogen peroxide; melanocytes; murine hair follicles;
D O I：
暂无
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学科分类号：
摘要：
Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. This is particularly evident in hair follicles. This paper reports on a serendipitous modification to an existing method which results in a drastically reduced background fluorescence. Immediately after antigen retrieval, sections exposed to 0.3% hydrogen peroxide in methanol for 30 min at room temperature exhibited low background fluorescence, increased antigenicity and revealed quantifiable numbers of melanocytes. This method is applicable to both human and mouse melanocytes particularly in the hair follicle.
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页码：1 / 3
页数：2
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