Complete Amino Acid Sequence of a Subunit from Rapeseed Protein

被引：0
作者：
Ravi Bhushan
Anil K. Tyagi
机构：
[1] University of Roorkee,Department of Chemistry
来源：
Journal of Plant Biochemistry and Biotechnology | 1998年 / 7卷
关键词：
amino acid sequence; protein subunit; rapeseed;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A subunit of molecular weight 18300 has been separated and isolated from seeds of Brassica campestris L. This subunit was cleaved by using cyanogen bromide, trypsin, Staphylococcus aureus V8 protease and chymotrypsin; the fragments obtained from enzymatlc and chemical cleavages were separated and isolated by polyacrylamide gel electrophoresis and gel filtration. The amino acid analyses were carried out. The complete amino acid sequence of the subunit containing 172 amino acid residues has been established by manual Edman method.
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页码：13 / 21
页数：8
相关论文
共 88 条
[1]  
Ohlson R.(1979)undefined J Am Oil Chem Soc 56 431-undefined
[2]  
Anjou K.(1983)undefined J Mol Appl Genet 2 273-undefined
[3]  
Crouch M.L.(1985)undefined Plant Mol Biol 5 191-undefined
[4]  
Tenbarge K.M.(1978)undefined J Biol. Chem 262 12196-undefined
[5]  
Simon A.E.(1987)undefined J Biol Chem 262 12202-undefined
[6]  
Ferl R.(1972)undefined Can J Plant Sci 52 63-undefined
[7]  
Simon A.E.(1976)undefined Phytochemistry 15 3-undefined
[8]  
Tenbarge K.M.(1984)undefined Proc Indian Acad Sci Chem Sci 93 1205-undefined
[9]  
Scofield S.R.(1972)undefined Biochim Biophys Acta 278 175-undefined
[10]  
Finkelstein R.R.(1990)undefined Int J Peptide Protein Res 35 310-undefined
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