A cytosolic NADP-malic enzyme gene from rice (Oryza sativa L.) confers salt tolerance in transgenic Arabidopsis

被引：0
|
作者：
Yuxiang Cheng
Mei Long
机构：
[1] Northeast Forestry University,Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology Laboratory
来源：
Biotechnology Letters | 2007年 / 29卷
关键词：
NADP-malic enzyme; Rice (; L.); Salt stress;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) functions in many different pathways in plant and may be involved in plant defense such as wound and UV-B radiation. Here, expression of the gene encoding cytosolic NADP-ME (cytoNADP-ME, GenBank Accession No. AY444338) in rice (Oryza sativa L.) seedlings was induced by salt stress (NaCl). NADP-ME activities in leaves and roots of rice also increased in response to NaCl. Transgenic Arabidopsis plants over-expressing rice cytoNADP-ME had a greater salt tolerance at the seedling stage than wild-type plants in MS medium-supplemented with different levels of NaCl. Cytosolic NADPH/NADP+ concentration ratio of transgenic plants was higher than those of wild-type plants. These results suggest that rice cytoNADP-ME confers salt tolerance in transgenic Arabidopsis seedlings.
引用
收藏
页码：1129 / 1134
页数：5
相关论文
共 50 条
  • [1] A cytosolic NADP-malic enzyme gene from rice (Oryza sativa L.) confers salt tolerance in transgenic Arabidopsis
    Cheng, Yuxiang
    Long, Mei
    BIOTECHNOLOGY LETTERS, 2007, 29 (07) : 1129 - 1134
  • [2] Expression of an NADP-malic enzyme gene in rice (Oryza sativa. L) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance
    Shenkui Liu
    Yuxiang Cheng
    Xinxin Zhang
    Qingjie Guan
    Shunsaku Nishiuchi
    Kenichi Hase
    Tetsuo Takano
    Plant Molecular Biology, 2007, 64 : 49 - 58
  • [3] Expression of an NADP-malic enzyme gene in rice (Oryza sativa. L) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance
    Liu, Shenkui
    Cheng, Yuxiang
    Zhang, Xinxin
    Guan, Qingjie
    Nishiuchi, Shunsaku
    Hase, Kenichi
    Takano, Tetsuo
    PLANT MOLECULAR BIOLOGY, 2007, 64 (1-2) : 49 - 58
  • [4] Salt tolerance of transgenic rice (Oryza sativa L.) with mtlD gene and gutD gene
    Wang, HZ
    Huang, DN
    Lu, RF
    Liu, JJ
    Qian, Q
    Peng, XX
    CHINESE SCIENCE BULLETIN, 2000, 45 (18): : 1685 - 1690
  • [5] Overexpression of Arabidopsis XERICO gene confers enhanced drought and salt stress tolerance in rice (Oryza Sativa L.)
    Zeng, De-Er
    Hou, Pei
    Xiao, Fangming
    Liu, Yongsheng
    JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2015, 24 (01) : 56 - 64
  • [6] Overexpression of Arabidopsis XERICO gene confers enhanced drought and salt stress tolerance in rice (Oryza Sativa L.)
    De-Er Zeng
    Pei Hou
    Fangming Xiao
    Yongsheng Liu
    Journal of Plant Biochemistry and Biotechnology, 2015, 24 : 56 - 64
  • [7] Salt tolerance of transgenic rice (Oryza sativa L.) with mtlD gene and gutD gene
    WANG Huizhong
    2. China National Rice Research Institute
    3. Institute of Microbiology
    ChineseScienceBulletin, 2000, (18) : 1685 - 1690
  • [8] Expression, purification, and characterization of two NADP-malic enzymes of rice (Oryza sativa L.) in Escherichia coli
    Cheng, YX
    Takano, T
    Zhang, XX
    Yu, S
    Liu, DL
    Liu, SK
    PROTEIN EXPRESSION AND PURIFICATION, 2006, 45 (01) : 200 - 205
  • [9] Overexpression of chloroplastic Zea mays NADP-malic enzyme (ZmNADP-ME) confers tolerance to salt stress in Arabidopsis thaliana
    Deepika Kandoi
    Baishnab C. Tripathy
    Photosynthesis Research, 2023, 158 : 57 - 76
  • [10] Overexpression of chloroplastic Zea mays NADP-malic enzyme (ZmNADP-ME) confers tolerance to salt stress in Arabidopsis thaliana
    Kandoi, Deepika
    Tripathy, Baishnab C.
    PHOTOSYNTHESIS RESEARCH, 2023, 158 (01) : 57 - 76
12345