Limited copy number - high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies

[1] Sharma SV(2007)Epidermal growth factor receptor mutations in lung cancer Nat Rev Cancer 7 169-181

[2] Bell DW(2005)Biomarkers in cancer staging, prognosis and treatment selection Nat Rev Cancer 5 845-856

[3] Settleman J(2008)Intratumor heterogeneity of epidermal growth factor receptor mutations in lung cancer and its correlation to the response to gefitinib Cancer Sci 99 929-935

[4] Haber DA(1998)Validation of a Method for Automated Nucleotide Sequence Analysis and Estimation of the Limits of Detection of Variant Sequences in a Heterogenic DNA Sample Analytical Letters 31 41-54

[5] Ludwig JA(2005)EGFR mutations in non-small-cell lung cancer: analysis of a large series of cases and development of a rapid and sensitive method for diagnostic screening with potential implications on pharmacologic treatment J Clin Oncol 23 857-865

[6] Weinstein JN(2005)Epidermal growth factor receptor gene mutation in non-small cell lung cancer using highly sensitive and fast TaqMan PCR assay Lung Cancer 50 375-384

[7] Taniguchi K(2006)Detection of epidermal growth factor receptor mutations in serum as a predictor of the response to gefitinib in patients with non-small-cell lung cancer Clin Cancer Res 12 3915-3921

[8] Okami J(2006)A rapid and sensitive enzymatic method for epidermal growth factor receptor mutation screening Clin Cancer Res 12 751-758

[9] Kodama K(2005)Sensitive sequencing method for KRAS mutation detection by Pyrosequencing J Mol Diagn 7 413-421

[10] Higashiyama M(2006)Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing Nat Med 12 852-855

[1] Sharma SV(2007)Epidermal growth factor receptor mutations in lung cancer Nat Rev Cancer 7 169-181

[2] Bell DW(2005)Biomarkers in cancer staging, prognosis and treatment selection Nat Rev Cancer 5 845-856

[3] Settleman J(2008)Intratumor heterogeneity of epidermal growth factor receptor mutations in lung cancer and its correlation to the response to gefitinib Cancer Sci 99 929-935

[4] Haber DA(1998)Validation of a Method for Automated Nucleotide Sequence Analysis and Estimation of the Limits of Detection of Variant Sequences in a Heterogenic DNA Sample Analytical Letters 31 41-54

[5] Ludwig JA(2005)EGFR mutations in non-small-cell lung cancer: analysis of a large series of cases and development of a rapid and sensitive method for diagnostic screening with potential implications on pharmacologic treatment J Clin Oncol 23 857-865

[6] Weinstein JN(2005)Epidermal growth factor receptor gene mutation in non-small cell lung cancer using highly sensitive and fast TaqMan PCR assay Lung Cancer 50 375-384

[7] Taniguchi K(2006)Detection of epidermal growth factor receptor mutations in serum as a predictor of the response to gefitinib in patients with non-small-cell lung cancer Clin Cancer Res 12 3915-3921

[8] Okami J(2006)A rapid and sensitive enzymatic method for epidermal growth factor receptor mutation screening Clin Cancer Res 12 751-758

[9] Kodama K(2005)Sensitive sequencing method for KRAS mutation detection by Pyrosequencing J Mol Diagn 7 413-421

[10] Higashiyama M(2006)Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing Nat Med 12 852-855