Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

被引：38

作者：

Ho Ko J. ^{[1
]}

Lee C.-S. ^{[2
]}

Kim K.H. ^{[3
]}

Pang M.-G. ^{[3
]}

Koo J.S. ^{[1
]}

Fang N. ^{[2
,4
]}

Koo D.-B. ^{[2
]}

Oh K.B. ^{[2
]}

Youn W.-S. ^{[2
]}

Zheng G.D. ^{[2
,5
]}

Park J.S. ^{[2
]}

Kim S.J. ^{[2
]}

Han Y.-M. ^{[2
]}

Choi I.Y. ^{[6
]}

Lim J. ^{[7
]}

Shin S.T. ^{[7
]}

Jin S.W. ^{[6
]}

Lee K.-K. ^{[2
]}

Yoo O.J. ^{[1
,3
]}

机构：

[1] Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yusong-gu, Taejon 305-701

[2] Korea Research Institute of Bioscience and Biotechnology, Taeduck Science Town, Taejon 305-600

[3] BioMedical Research Center, Korea Advanced Institute of Science and Technology, Yusong-gu, Taejon 305-701

[4] Department of Animal Science, College of Agriculture, Yanbian University

[5] Department of Anatomy, College of Medicine, Yanbian University

[6] Central Research Institute, Hanmi Pharmaceutical Company, Ltd., Sungnam, Kyunggi-do 463-400, Sampyong-dong

[7] College of Veterinary Medicine, Chungnam National University, Yusong-gu, Taejon 305-764, Kung-dong

来源：

Transgenic Research | 2000年 / 9卷 / 3期

关键词：

Biological activity; Bioreactor; G-CSF; O-glycosylation; Transgenic goat;

D O I：

10.1023/A:1008972010351

中图分类号：

学科分类号：

摘要：

We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.

引用

页码：215 / 222

页数：7

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