Online immunocapture ICP-MS for the determination of the metalloprotein ceruloplasmin in human serum

被引：13

作者：

Bernevic B. ^{[1
]}

El-Khatib A.H. ^{[2
,3
]}

Jakubowski N. ^{[2
]}

Weller M.G. ^{[1
]}

机构：

[1] Division 1.5 Protein Analysis, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Strasse 11, Berlin

[2] Division 1.1 Inorganic Trace Analysis, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Strasse 11, Berlin

[3] Faculty of Pharmacy, Department of Analytical Chemistry, Ain Shams University, Organization of African Unity Street, Abassia Cairo

来源：

BMC Research Notes | / 11卷 / 1期

关键词：

Ceruloplasmin; ELISA; Human serum; ICP-MS; Immunocapture;

D O I：

10.1186/s13104-018-3324-7

中图分类号：

学科分类号：

摘要：

Objective: The human copper-protein ceruloplasmin (Cp) is the major copper-containing protein in the human body. The accurate determination of Cp is mandatory for the reliable diagnosis of several diseases. However, the analysis of Cp has proven to be difficult. The aim of our work was a proof of concept for the determination of a metalloprotein-based on online immunocapture ICP-MS. The immuno-affinity step is responsible for the enrichment and isolation of the analyte from serum, whereas the compound-independent quantitation with ICP-MS delivers the sensitivity, precision, and large dynamic range. Off-line ELISA (enzyme-linked immunosorbent assay) was used in parallel to confirm the elution profile of the analyte with a structure-selective method. The total protein elution was observed with the 32S mass trace. The ICP-MS signals were normalized on a 59Co signal. Results: The human copper-protein Cp could be selectively determined. This was shown with pure Cp and with a sample of human serum. The good correlation with off-line ELISA shows that Cp could be captured and eluted selectively from the anti-Cp affinity column and subsequently determined by the copper signal of ICP-MS. © 2018 The Author(s).

引用

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