MiRNA Profile in the Substantia Nigra of Parkinson’s Disease and Healthy Subjects

被引：0
作者：
Lucía F. Cardo
Eliecer Coto
René Ribacoba
Manuel Menéndez
Germán Moris
Esther Suárez
Victoria Alvarez
机构：
[1] Hospital Universitario Central de Asturias,Genética Molecular
[2] Hospital Universitario Central Asturias and Álvarez Buylla-Mieres,Laboratorio de Medicina
来源：
Journal of Molecular Neuroscience | 2014年 / 54卷
关键词：
Parkinson’s disease; MicroRNAs; Substantia nigra; RNA expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The deregulation of several microRNAs (miRNAs) has been associated with neurodegenerative processes, including Parkinson’s disease (PD). Our aim was to characterize the level of miRNAs in the substantia nigra (SN) of PD patients and healthy donors. This is an important issue to characterize new putative markers and therapeutic targets for PD. RNA was extracted from the SN of postmortem PD (n = 8) and healthy (n = 4) subjects, and the level of 733 human miRNAs was assayed with TaqMan low-density arrays (TLDAs). Overall, there was a miRNA downregulation in the SN of patients. The mean level of 11 miRNAs was significantly different (p < 0.05) between patients and controls, with 10 downregulated among the patients. MiR-198, -135b, -485-5p, and -548d were the best candidates and were quantified with individual TaqMan assays in the 12 samples. MiR-135b showed the most significant difference between patients and healthy donors. The bioinformatic analysis suggested that this miRNA could bind to genes implicated in several neurodegenerative pathways.
引用
收藏
页码：830 / 836
页数：6
相关论文
共 125 条
  • [11] Morato X(2010)Post-transcriptional regulation of alpha-synuclein expression by mir-7 and mir-153 J Biol Chem 285 12726-641
  • [12] Compta Y(2009)Understanding microRNAs in neurodegeneration Nat Rev Neurosci 10 837-1006
  • [13] Braak H(2010)Evidence for natural antisense transcript-mediated inhibition of microRNA function Genome Biol 11 R56-13057
  • [14] Ghebremedhin E(2010)Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression Nature 466 637-331
  • [15] Rub U(2008)Serum microRNAs are promising novel biomarkers PLoS One 3 e3148-1224
  • [16] Bratzke H(2007)Brain expressed microRNAs implicated in schizophrenia etiology PLoS One 2 e873-858
  • [17] Del Tredici K(2010)Robust microRNA stability in degraded RNA preparations from human tissue and cell samples Clin Chem 56 998-5198
  • [18] Cardo LF(2009)Repression of alpha-synuclein expression and toxicity by microRNA-7 Proc Natl Acad Sci U S A 106 13052-3078
  • [19] Coto E(2012)Plasma-based circulating microRNA biomarkers for Parkinson’s disease J Park Dis 2 321-1106
  • [20] de Mena L(2007)A MicroRNA feedback circuit in midbrain dopamine neurons Science 317 1220-780
12345678910