MiRNA Profile in the Substantia Nigra of Parkinson’s Disease and Healthy Subjects

被引：0
作者：
Lucía F. Cardo
Eliecer Coto
René Ribacoba
Manuel Menéndez
Germán Moris
Esther Suárez
Victoria Alvarez
机构：
[1] Hospital Universitario Central de Asturias,Genética Molecular
[2] Hospital Universitario Central Asturias and Álvarez Buylla-Mieres,Laboratorio de Medicina
来源：
Journal of Molecular Neuroscience | 2014年 / 54卷
关键词：
Parkinson’s disease; MicroRNAs; Substantia nigra; RNA expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The deregulation of several microRNAs (miRNAs) has been associated with neurodegenerative processes, including Parkinson’s disease (PD). Our aim was to characterize the level of miRNAs in the substantia nigra (SN) of PD patients and healthy donors. This is an important issue to characterize new putative markers and therapeutic targets for PD. RNA was extracted from the SN of postmortem PD (n = 8) and healthy (n = 4) subjects, and the level of 733 human miRNAs was assayed with TaqMan low-density arrays (TLDAs). Overall, there was a miRNA downregulation in the SN of patients. The mean level of 11 miRNAs was significantly different (p < 0.05) between patients and controls, with 10 downregulated among the patients. MiR-198, -135b, -485-5p, and -548d were the best candidates and were quantified with individual TaqMan assays in the 12 samples. MiR-135b showed the most significant difference between patients and healthy donors. The bioinformatic analysis suggested that this miRNA could bind to genes implicated in several neurodegenerative pathways.
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页码：830 / 836
页数：6
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  • [1] Alvarez-Erviti L(2013)Influence of microRNA deregulation on chaperone-mediated autophagy and alpha-synuclein pathology in Parkinson’s disease Cell Death Dis 4 e545-466
  • [2] Seow Y(2013)MicroRNA in Alzheimer’s disease: an exploratory study in brain, cerebrospinal fluid and plasma Biomarkers 18 455-1077
  • [3] Schapira AH(2014)Identification of blood serum micro-RNAs associated with idiopathic and LRRK2 Parkinson’s disease J Neurosci Res 92 1071-134
  • [4] Rodriguez-Oroz MC(2004)Stages in the development of Parkinson’s disease-related pathology Cell Tissue Res 318 121-1422
  • [5] Obeso JA(2013)Profile of microRNAs in the plasma of Parkinson’s disease patients and healthy controls J Neurol 260 1420-49
  • [6] Cooper JM(2014)Alpha-synuclein transcript isoforms in three different brain regions from Parkinson’s disease and healthy subjects in relation to the SNCA rs356165/rs11931074 polymorphisms Neurosci Lett 562 45-348
  • [7] Bekris LM(2014)The screening of the 3′UTR sequence of LRRK2 identified an association between the rs66737902 polymorphism and Parkinson’s disease J Hum Genet 59 346-620
  • [8] Lutz F(2014)Studying the system-level involvement of microRNAs in Parkinson’s disease PLoS One 9 e93751-2033
  • [9] Montine TJ(2012)MicroRNA-205 regulates the expression of Parkinson’s disease-related leucine-rich repeat kinase 2 protein Hum Mol Genet 22 608-12734
  • [10] Botta-Orfila T(2013)No differential DNA methylation of PARK2 in brain of Parkinson’s disease patients and healthy controls Mov Disord 28 2032-841
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