Assessment of genetic diversity in Coho salmon (Oncorhynchus kisutch) populations with no family records using ddRAD-seq

被引：22

作者：

Hosoya S. ^{[1
]}

Kikuchi K. ^{[1
]}

Nagashima H. ^{[2
]}

Onodera J. ^{[2
]}

Sugimoto K. ^{[2
]}

Satoh K. ^{[2
]}

Matsuzaki K. ^{[2
]}

Yasugi M. ^{[3
]}

Nagano A.J. ^{[3
,4
,5
]}

Kumagayi A. ^{[2
]}

Ueda K. ^{[2
]}

Kurokawa T. ^{[6
]}

机构：

[1] Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, University of Tokyo, 2971-4 Bentenjima, Maisaka Hamamatsu Shizuoka

[2] Miyagi Prefecture Fisheries Technology Institute, Freshwater Fisheries Experimental Station, Taiwa Miyagi

[3] Center for Ecological Research, Kyoto University, Hirano 509-3-2, Otsu Shiga

[4] JST CREST, Honcho 4-1-8, Kawaguchi Saitama

[5] Faculty of Agriculture, Ryukoku University, Yokotani 1-5, Seta Ohe-cho Otsu-shi Shiga

[6] Kushiro Laboratory, Hokkaido National Fisheries Research Institute, Japan Fisheries Research and Education Agency, 116 Katsurakoi, Kushiro-shi Hokkaido

来源：

BMC Research Notes | / 11卷 / 1期

关键词：

Breeding value; Coho salmon (Oncorhynchus kisutch); ddRAD-seq; Genetic diversity; Genomic best linear unbiased prediction; Heritability; Prediction accuracy; Selective breeding; SNPs;

D O I：

10.1186/s13104-018-3663-4

中图分类号：

学科分类号：

摘要：

Objective: Selective breeding for desirable traits is becoming popular in aquaculture. In Miyagi prefecture, Japan, a selectively bred population of Coho salmon (Oncorhynchus kisutch) has been established with the original, randomly breeding population maintained separately. Since they have been bred without family records, the genetic diversity within these populations remains unknown. In this study, we estimated the genetic diversity and key quantitative genetic parameters such as heritability and genomic breeding value for body size traits by means of genomic best linear unbiased prediction to assess the genetic health of these populations. Results: Ninety-nine and 83 females from the selective and random groups, respectively, were genotyped at 2350 putative SNPs by means of double digest restriction associated DNA sequencing. The genetic diversity in the selectively bred group was low, as were the estimated heritability and prediction accuracy for length and weight (h 2 = 0.26-0.28; accuracy = 0.34), compared to the randomly bred group (h 2 = 0.50-0.60; accuracy = 0.51-0.54). Although the tested sample size was small, these results suggest that further selection is difficult for the selectively bred population, while there is some potential for the randomly bred group, especially with the aid of genomic information. © 2018 The Author(s).

引用

共 16 条

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